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New use of tripterine in pharmacy

A technology of tripterine and medicine, which is applied in the field of medicine, can solve the problems of anti-inflammation and anti-oxidation, etc., and achieve the effect of significant cytotoxicity and effective anti-tumor proliferation

Inactive Publication Date: 2009-01-28
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of progress has been made in the research of tripterygium, the exact molecular mechanism of its anti-inflammation, anti-oxidation and anti-cancer effects has not been fully explained. In addition, whether tripteryne can be used to treat patients expressing CIP2A protein Cancers such as lung cancer, liver cancer, gastric cancer, breast cancer and other high-incidence cancers or the way to treat the above-mentioned various cancers have not been reported

Method used

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  • New use of tripterine in pharmacy
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  • New use of tripterine in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Tripteryglide (5μM) treated various logarithmic growth phase lung cancer cells (A549cells, HCC827cells, NCI-H1975 cells, NCI-H460 cells), breast cancer cell lines (MCF-7 cells), gastric cancer cell lines (SGC- 7901 cells), liver cancer cell line (HepG-2), drug action time according to figure 2As shown, then collect cells with RIPA lysate (50mM Tris (pH7.4), 150mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% SDS, Protease Inhibitor Cocktail, 1mMPMSF, 1mM NaF, 1Mm Na3VO4, 1Mm EDTA) lysed cells to extract protein, and carried out Western blot immunoblotting experiment with anti-CIP2A mouse monoclonal antibody 2G10-3B5 (purchased from Santa Cruz Biotechnology Company). CIP2A protein can be rapidly and significantly down-regulated without cell line specificity. see figure 1 .

Embodiment 2

[0035] Tripterygium (5μM) treated the lung cancer cell line A549 in the logarithmic growth phase, and the drug action time was according to figure 2 As shown, the collected cells were lysed with RIPA lysate to extract protein, and then Western blot was performed with the anti-CIP2A mouse monoclonal antibody 2G10-3B5 (purchased from Santa Cruz Biotechnology Company). The results showed that Tripterygium wilfordii can Time-dependent downregulation of CIP2A protein. see figure 2 .

Embodiment 3

[0037] The lung cancer cell line A549 was treated with tripteryglide at various concentrations, and the drug action time was 6 hours. The cells were collected and lysed with RIPA lysate to extract the protein, and then the anti-CIP2A mouse monoclonal antibody 2G10-3B5 (purchased from Santa Cruz Biotechnology company) performed Western blot immunoblotting experiments, and the results showed that tripterygium can down-regulate CIP2A protein in a dose-dependent manner. see image 3 .

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Abstract

The invention discloses the application of celastrol in the preparation of the medicine for treating cancer expressing CIP2A protein. The celastrol of the invention has the characteristics of having no obvious down regulation CIP2A protein with the cell line particularity, reducing the expressing of oncoprotein in lung cancer, liver cancer, breast cancer and gastric cancer with time and dose pendence, causing oncoprotein C-Myc steadied by CIP2A to generate corresponding retrogradation, displaying remarkable cytotoxic activity in the cell of lung cancer and liver cancer, and having effective function of anti-tumor proliferation in the transplant model of tumor of nude mice lung cancer. The celastrol has a wide application prospect in treating lung cancer, head and neck squamous cell carcinoma, carcinoma of colon, gastric cancer, liver cancer, breast cancer and other cancer expressing CIP2A oncoprotein.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the new application of tripterine in pharmacy. Background technique [0002] Cancer is an extremely complex and diverse disease. About 1 / 8 of the world's population deaths are caused by cancer, which exceeds the sum of the deaths of AIDS, tuberculosis and malaria. In developed countries, cancer is the second largest after heart disease. It is the third leading cause of death in developing countries. Almost all cancers have the same characteristics during the development process. In summary, they are mainly reflected in the following aspects: self-sustaining growth signals, insensitivity to growth inhibitory signals, evasion of apoptosis, unlimited replication potential, continuous angiogenesis and acquisition of Tissue invasion and metastasis ability. Investigating its etiology, cancer is mainly caused by internal and external factors. External factors include smoking, chemi...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P35/00
Inventor 周光飚马亮吴福群刘永强刘姿
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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