Reagent kit for detecting children asthma genetic susceptibility

A kit and susceptibility technology, applied in the field of kits for detecting the genetic susceptibility of childhood asthma, can solve the problems of complex and unexplainable causes of allergic asthma.

A kit and susceptibility technology, applied in the field of kits for detecting the genetic susceptibility of childhood asthma, can solve the problems of complex and unexplainable causes of allergic asthma.

CN101354345AInactive Publication Date: 2009-01-28XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] A fluorescent quantitative PCR detection kit for detecting genetic susceptibility to asthma in children is used, which contains the following primer pairs and fluorescent probe pairs:

[0032] Sense primer 1: 5'-CAATCCTGTGTTTGTTAGTTGTGCTAT-3' (SEQ ID NO: 1)

[0033] Antisense primer 1: 5'-GATTTGCACAGCAAATTGAGTTAGA-3' (SEQ ID NO: 2)

[0034] Sense primer 2: 5'-TCATTAACCTCGTTCCAACATGTAA-3' (SEQ ID NO: 3)

[0035] Antisense primer 2: 5'-CGGACTCCAGTGAGCCATAATT-3' (SEQ ID NO: 4)

[0036] Sense primer 3: 5'-CCATCATGTCTCCCTCTCCTTTTCTAGT-3' (SEQ ID NO: 5)

[0037] Antisense primer 3: 5'-TGTTCCAACCCTGCATGCT-3' (SEQ ID NO: 6)

[0038] Sense primer 4: 5'-GGAGAGATCCCTGTGTTGCAATAT-3' (SEQ ID NO: 7)

[0039] Antisense primer 4: 5'-CAGGCTGTTTCCTCTTGTGATGT-...

Embodiment 2

[0066] Example 2. Services for testing people's genetic susceptibility to asthma in children

[0067] Step 1: DNA Extraction

[0068] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0069] Step 2: Genotyping Assays

[0070] Using the kit provided by the present invention, the SNP site No. rs545861 on the ADAMTS12 gene, the SNP site No. rs3901901 on the ELF5 gene, the SNP site No. rs8193036 on the IL-17 gene, and the SNP site No. The rs2279776 SNP site, the rs2175595 SNP site and the rs10815925 SNP site were detected by fluorescent quantitative PCR to determine the genotypes of the six SNP sites.

[0071] Step 3: Analysis of genetic susceptibility to asthma in children

[0072] Through the analysis of the SNP genotype of the tested subjects, an analysis report sheet of the genetic susceptibility of c...

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Abstract

The invention discloses a kit that is used for detecting the genetic predisposition of children asthma. The kit comprises particularity primer pairs and particularity fluorescent probe pairs that are used for simultaneously detecting six SNP loci on the four genes of dissociation agent-metalloproteinase (ADAMTS12) that has the encoding of thrombin-sensitive protein motif 12, E74-like factor 5 (ELF5), interleukin-17 (IL-17) and receptor-typed protein tyrosine phosphatase D (PTPRD) and a routine component that is used for fluorescent quantitative PCR detection, etc. The kit of the invention evaluates the genetic predisposition of children asthma by simultaneously detecting the mononucleotide polymorphism locus genotypes that are closely related with the genetic predisposition of children asthma.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting the genetic susceptibility of children's asthma, by simultaneously detecting the coded gene closely related to the genetic susceptibility of children's asthma with the thrombin sensitive protein motif 12 The single nucleotide polynucleotides of the four genes of dissociin-metalloproteinase (ADAMTS12), E74-like factor 5 (ELF5), interleukin-17 (IL-17) and receptor-type protein tyrosine phosphatase D (PTPRD) To assess the genetic susceptibility of children with asthma by using the genotype of morphological loci. Background technique [0002] Allergic asthma (asthma) is a common disease in children in my country, clinically manifested as recurrent wheezing, shortness of breath, chest tightness and / or coughing and other symptoms, mostly at night or early morning. The prevalence rate in our country is about 1...

Claims

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Application Information

Patent Timeline
28 Jan 2009
Publication
CN101354345A
IPC
G01N21/00; G01N33/50; C12Q1/68
Inventors
冯哲民; 邹祖烨