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Medium pollen, preparation method and use thereof

A kind of medium pollen, pollen technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., to achieve good economic and social benefits, ensure seed quality, and improve the effect of effective utilization

Active Publication Date: 2009-02-04
YUXI ZHONGYAN TOBACCO SEED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, tobacco anthers and pollen have homology, if anthers are used as pollen extender to prepare medium pollen, it will not have negative impact on pollen viability, and should be able to achieve better results, but this has not yet been seen in the prior art. There are relevant reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The selected demonstration field is located in the seed production base of China Tobacco Seed Co., Ltd. in Yuxi, Xishuangbanna, Yunnan. Tobacco seedlings are raised by floating seedlings, which are then transplanted to the field after they grow into seedlings. Field planting density: row spacing 1.2m, plant spacing 0.5m.

[0019] Field implementation of 10 mu in the seed production base. Among them, 2 mu are used as the male parent of MS Yunyan 87, and the remaining 8 mu are used for planting the female parent of MS Yunyan 87. The anthers of the male parent of MS Yunyan 87 were collected in the field, dried to loosen the powder, and the pollen and anther sacs were screened out with a 50-mesh sieve, and the pollen was collected, sealed and stored at 4°C for later use. Collected pollen sacs were dried at 40°C for 24 hours, ground into powder, and sealed and stored at 4°C for later use. Mix the anther powder with the pure pollen of the male parent of MS Yunyan 87 at a v...

Embodiment 2

[0021] Field implementation of 10 mu in the seed production base. Among them, 2 mu are used as the male parent field of MSK326, and the remaining 8 mu are used for planting the female parent of MSK326. The anthers of the male parent of MSK326 were collected in the field, dried to loosen the powder, the pollen and anther sacs were screened out with a 50-mesh sieve, the pollen was collected, and sealed and stored at 4°C for later use. Collected pollen sacs were dried at 40°C for 24 hours, ground into powder, and sealed and stored at 4°C for later use. Mix the pollen sac powder with the pure pollen of the male parent of MSK326 uniformly at a volume ratio of 1:2 to prepare the required medium pollen.

Embodiment 3

[0023] Field implementation of 10 mu in the seed production base. Among them, 2 mu are used as the male parent of MS Yunyan 85, and the remaining 8 mu are used for planting the female parent of MS Yunyan 85. The anthers of the male parent of MS Yunyan 85 were collected in the field, dried to loosen the powder, and the pollen and anther sacs were screened out with a 50-mesh sieve, and the pollen was collected and sealed and stored at 4°C for later use. Collected pollen sacs were dried at 40°C for 24 hours, ground into powder, and sealed and stored at 4°C for later use. Mix the anther powder and the pure pollen of the male parent of MS Yunyan 85 uniformly at a volume ratio of 1:2 to prepare the required medium pollen.

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Abstract

The invention discloses a medium pollen, a preparation method and the application thereof. The preparation method is as follows: tobacco male parent anther is collected and aired to cause the anther to loose power, a 50 mesh sieve is used for sieving out pollen and pollen sac, the pollen sac is collected, dried at 40-50 DEG C for 20-24 hours, and ground into powder; the pollen sac powder and the pure tobacco pollen are mixed evenly according to 1:1-4 of volume ratio, and the required medium pollen is obtained. The medium pollen is dipped by a cotton swab or a brush pen and applied to the female parent tobacco flower column head for artificial pollination. The use of the medium pollen improves effective utilization ratio of the tobacco pollen, decreases application amount of the pure pollen, saves manpower and material resources for collecting pollen, decreases planting area of the tobacco male parents, and lowers production cost of seed production by hybridization. The popularization and application of the preparation method thereof have good economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of artificial pollination, and relates to artificial assisted pollination for tobacco hybrid seed production, in particular to medium pollen prepared by utilizing tobacco anther sacs. At the same time, the invention also relates to the preparation method and application of the medium pollen. Background technique [0002] Artificial pollination is an important and indispensable work in the hybrid seed production of tobacco male sterile lines. The amount of pollen in the tobacco male parent is small and difficult to collect. Pollen collection in production is a very expensive task. At present, pure tobacco pollen is used for artificial pollination in the process of hybrid seed production of tobacco sterile lines, but this method consumes a large amount of pollen, the effective utilization rate of pollen is low, and the cost is too high. my country's tobacco sterile line varieties have a large planting area a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 马文广郑昀晔李永平邓盛斌陈云松牛永志索文龙宋碧清陈连红
Owner YUXI ZHONGYAN TOBACCO SEED
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