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Trace amount terminal colour development limulus experiment method

A technology of limulus test and chromogenic method, which is applied in the field of trace end-point chromogenic limulus test, can solve the problems of the pharmaceutical industry's attack and the shortage of limulus reagent supply, and achieve the effect of reduced dosage and simple and fast experimental operation

Active Publication Date: 2009-02-18
湛江安度斯生物有限公司
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Problems solved by technology

If the supply of Limulus reagent is in short supply due to the depletion of Limulus resources, it will be a catastrophic blow to my country's pharmaceutical industry

Method used

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  • Trace amount terminal colour development limulus experiment method
  • Trace amount terminal colour development limulus experiment method
  • Trace amount terminal colour development limulus experiment method

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Embodiment Construction

[0089] For example: the method and steps for bacterial endotoxin inspection of a test product using the micro-endpoint chromogenic Limulus test are as follows.

[0090] 1. Prepare the limulus reagent (TAL) used in the end-point chromogenic limulus test.

[0091] 2. Preparation of the chromogenic matrix used in the end-point chromogenic limulus test.

[0092] 3. Prepare bacterial endotoxin test water (BET water).

[0093] 4. Manufacture a plastic 96-well microwell microplate 1 (Microplate), the diameter of each hole 2 of the microplate is Φ3.0-5.0 mm, the hole depth is 7-12 mm, the outer circumference of the microplate and the size of each hole The center position is the same as the peripheral size of the microplate plate and the center position of each well in Figure 1-Well Positions of a 96 well microplate in the standard ANSI / SBS 4-2004 for Microplates-WellPositions of the American National Standards Institute and the Society for Biomolecular Screening.

[0094] 5. Prepare...

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Abstract

The invention relates to the bacterial endotoxin detecting technique, in particular to a minim endpoint colored limulus test method. The method uses enzyme label plate with 96 micro holes as the reaction vessel and photometry vessel, and uses trace LAL (Tachypleus Amebocyte Lysate, TAL or Limulus Amebocyte Lysate, LAL) as the endpoint colored method for the limulus test. The amount of TAL consumed in the method of trace amount endpoint colored limulus test method only equals to 10 to 20 percent of that consumed in the current limulus test method, namely 10 to 20u 1 / T, and the test operation is more simple and swift. Part content in the test method can also be applied to other photometry limulus test, and consumption of ATL can be reduced by 80 to 90 percent.

Description

technical field [0001] The invention relates to a bacterial endotoxin detection technology, in particular to a micro-endpoint chromogenic limulus test method. Background technique [0002] Bacterial endotoxin detection technology has important application value in modern pharmaceutical industry. The current detection method for bacterial endotoxin is the limulus test. The limulus test method needs to use the limulus reagent (TAL or LAL), and the production of the limulus reagent needs to consume precious limulus resources. In my country, horseshoe crab resources are on the verge of exhaustion. At present, there is no more mature or better bacterial endotoxin detection method that can replace the Limulus test. If the supply of Limulus reagent is in short supply due to the depletion of Limulus resources, it will be a catastrophic blow to my country's pharmaceutical industry. Today, when there is no better alternative method, it is of great and practical significance to stu...

Claims

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Application Information

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IPC IPC(8): G01N33/579G01N21/79
Inventor 冯聚锦韦群
Owner 湛江安度斯生物有限公司
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