Melamine immune chromatography test paper detecting method
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An immunochromatographic test paper, melamine technology, applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of high price, expensive equipment, narrow scope of application, etc., and achieve the effect of simple and convenient operation
Inactive Publication Date: 2009-03-18
WUHAN POLYTECHNIC UNIVERSITY
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These instrumental methods are expensive, and the methods are complicated to operate, requiring specially trained personnel to operate, and there are problems such as narrow scope of application, expensive equipment, and high cost.
At present, there is no method for detecting melamine in dairy products with strong specificity, high sensitivity, simple and convenient operation, suitable for rapid on-site detection and large-scale census in the technical field.
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[0032] Below in conjunction with accompanying drawing, the present invention is described in further detail:
[0033] 1 test line + 1 reference line test paper
[0034] The detection line is coated with melamine polyclonal antibody at a concentration of 0.1-30 μg / mL or 1 antigen for melamine detection, 1 mm wide; the reference line is coated with IgG against the second species of animal protein at a concentration of 0.1-30 μg / mL 1 line, 1mm wide; the interval between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection line, which can be greater than or equal to A certain concentration of 5 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is invalid.
[0035] Further ex...
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Abstract
The invention relates to a melamine immunity-chromatography test strip test method, comprising: the liner of the test strip is attached with a sample solution absorption part, an adhesive gold mark part, a test reaction part and a water adsorption part in turn, wherein the test reaction part is enveloped by one melamine multi-clone antibody or one detection antigen as the detection line and is enveloped by one IgG resisting the second animal protein as the reference line. The fast detection test strop has strong specificity, can realize half-quantitative detection, can be used in the environment temperature of 4 to 40 DEG C, is suitable for the fast test on the food and feeds of melamine pollution for milk product enterprises, individual cultivators, food health quality monitor departments, customs and supermarkets and the like. The melamine immunity-chromatography test strip test method has the advantages of strong specificity, high sensitivity, half-quantitative test and simple operation and the like.
Description
technical field [0001] The invention belongs to the field of food safety detection, and specifically relates to a detection method of harmful residues in food, in particular to a detection method of melamine test paper. Background technique [0002] Melamine (English name Melamine), referred to as triamine, also known as 2,4,6-triamino-1,3,5-triazine, 1,3,5-triazine-2,4,6-triamine, 2 , 4,6-trisaminocarbamide, melamine, melamine, and cyanuronamide are a triazine nitrogen-containing heterocyclic organic compound and an important nitrogen-heterocyclic organic chemical raw material. Melamine Molecular Formula C 3 N 6 h 6 、C 3 N 3 (NH 2 ) 3 , molecular weight 126.12. [0003] In the process of food production and sales, it is necessary to determine the protein content of food, but the direct measurement of protein is technically complicated, so the Kjeldahl method is usually used to determine the protein content, that is, the protein content can be indirectly calculated b...
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