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Visible light quantitative determination kit for nitroso mercaptan

A nitrosothiol, quantitative detection technology is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., to simplify the complicated steps and save the experimenter's time.

Inactive Publication Date: 2010-08-18
HUAZHONG NORMAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

At present, there is no kit available for the quantitative detection of SNO at home and abroad

Method used

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  • Visible light quantitative determination kit for nitroso mercaptan
  • Visible light quantitative determination kit for nitroso mercaptan

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Embodiment Construction

[0017] The composition of the present invention and the method of use are further described in detail in conjunction with the accompanying drawings:

[0018] The composition of the detection kit:

[0019] Reagent bottle 1: black plastic bottle, sealed inside the bottle and packed with sulfonamide solution dissolved in 5% phosphoric acid. Accurately weigh 1g of sulfonamide and fully dissolve it in 100mL of 5% phosphoric acid solution, this is reagent I.

[0020] Reagent bottle 2: a black plastic bottle, sealed inside the bottle and packed with sulfonamide solution in 5% phosphoric acid containing 3.7mM mercuric chloride. Accurately weigh 0.05g of mercuric chloride, dissolve it in 1mL of DMSO (dimethyl sulfoxide), and dissolve it together with 1g of sulfonamide in 100mL of 5% phosphoric acid solution. This is reagent II.

[0021] Reagent bottle 3: a black plastic bottle with 0.1% NED solution sealed inside the bottle. Accurately weigh 0.1g NED and dissolve it in 100mL double ...

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Abstract

The invention relates to a visible light quantitative detection reagent kit for s-nitrosothiol, which comprises five reagent bottles which are a reagent bottle 1, a reagent bottle 2, a reagent bottle 3, a reagent bottle 4 and a reagent bottle 5 respectively, a kit body (7), a kit cover (8), retaining plates provided with holes (6), and a reagent kit specification (9), wherein the five reagent bottles are separated and fixed through the retaining plates provided with holes (6); a descriptive label is arranged on each reagent bottle; and the accurately weighed reagents with fixed weight proportions are hermetically enclosed into the reagent bottles, wherein a sulfanilamide solution which is dissolved into 5 weight percent of phosphoric acid is filled into the reagent bottle 1; a sulfanilamide solution which is dissolved into 5 weight percent of phosphoric acid and contains mercuric chloride is filled into the reagent bottle 2; N-1-naphthylenediamine dihydrochloride is filled into the reagent bottle 3; a sulfosalicylic acid is filled into the reagent bottle 4; and a NaNO2 standard solution is filled into the reagent bottle 5. The reagent kit is simple, convenient and flexible to detect the s-nitrosothiol level of various cell samples.

Description

technical field [0001] The invention relates to a kit for quantitative detection of nitrosothiols, which is suitable for basic research on the etiology and pathogenesis of diseases related to nitrosothiols and nitric oxide signaling pathways, and is suitable for various environmental or chemical factors. Quantitative detection of changes in nitrosothiol levels. Background technique [0002] S-nitrosothiol (SNO) is an ester of nitric oxide and thiol compounds, which plays an important role in nitric oxide signaling pathway. The formation of SNO can preserve the biological activity of NO and regulate protein activity. Under physiological and pathological conditions, the SNO signaling pathway may be up-regulated or down-regulated. Therefore, nitrosothiols are very important as biomarkers for certain diseases the value of. [0003] At home and abroad, we have been working on effective SNO detection methods for many years. Some of the existing methods have low detection sensiti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 曹毅杨旭王昆丁书茂袁均林
Owner HUAZHONG NORMAL UNIV
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