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Reagent box for detecting content of protein carbonyl group

A technology for detecting kits and proteins, applied in biological testing, material inspection products, etc., can solve the problems of complex sample processing, multi-use, expensive reagents, etc., and achieve the effect of simple sample processing

Inactive Publication Date: 2007-01-17
HUAZHONG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In 1987, Oliver et al proposed that protein carbonyl content could be used as an index to judge protein oxidative damage. Over the years, domestic and foreign countries have been working on effective detection methods of protein carbonyl content to evaluate the degree of protein oxidative damage. Methods include sodium borohydride method, fluorescein hydrazine method, fluorescein amine method, immunohybridization method, etc. These methods either require expensive equipment or expensive reagents, and the sample processing is very complicated
The 2,4-dinitrophenylhydrazine (DNPH) colorimetric method is a classic method for determining protein carbonyl content. It is simple and effective in detecting protein carbonyl content. It is inconvenient, and there will be large systematic errors due to the accuracy of the experimenters and instruments, and some scholars at home and abroad have many deficiencies in designing experiments, which will affect the final experimental results
There is no domestic kit for detecting protein oxidative damage, and some foreign companies sell specific antibodies, but they are relatively expensive

Method used

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  • Reagent box for detecting content of protein carbonyl group
  • Reagent box for detecting content of protein carbonyl group

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Embodiment Construction

[0017] Below in conjunction with the composition of detection kit and accompanying drawing and method of use, the present invention is described in further detail:

[0018] The composition of the detection kit:

[0019] Reagent bottle 1: white plastic bottle, containing a solution prepared by dissolving phenylmethylsulfonyl fluoride in isopropanol. The concentration of phenylmethylsulfonyl fluoride is 20 mg / mL, and the volume is 1 mL. Dilute with reagent I before use Dilute it with liquid and use it as a homogenization medium. This is Reagent I.

[0020] Reagent bottle 2: white plastic bottle, filled with 10mmol / LHEPES, 137mmol / LNaCl, 4.6mmol / LKCl, 1.1mmol / L KH 2 PO 4 , 0.6mmol / L MgSO 4 , 0.5mg / L Aprotinin and 1.1mmol / L EDTA are prepared according to the fixed concentration of the solution, the pH value of the solution is 7.4, and the volume is 60mL. This is the reagent I dilution.

[0021] Reagent bottle 3: a white plastic bottle, containing a solution prepared by 50mol...

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Abstract

A reagent kit for detecting carbonyl content of protein in tissue organ, serum and cell consists of a set of seven bottles reagent used coordinately and used as a complete set in detection. Each bottle is packed with reagent in set concentration and set volume as bottle one is placed in foam box with ice bag and the others are placed in the same box made by hard paper-plastic material.

Description

Technical field [0001] The invention relates to a kit for detecting the carbonyl content of protein, which is suitable for detecting the degree of protein oxidation damage in various tissues, organs, serum, cultured cells and organelles. Background technique [0002] In 1987, Oliver et al proposed that protein carbonyl content could be used as an index to determine protein oxidative damage. Over the years, domestic and foreign countries have been working on effective detection methods for protein carbonyl content to evaluate the degree of protein oxidative damage. Methods include sodium borohydride method, fluorescein hydrazine method, fluorescamine method, immunoblotting method, etc. These methods either require expensive equipment or expensive reagents, and the sample processing is very complicated. The 2,4-dinitrophenylhydrazine (DNPH) colorimetric method is a classic method for the determination of protein carbonyl content. It is simple and effective in detecting protein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/52
Inventor 段丽菊杨旭
Owner HUAZHONG NORMAL UNIV
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