Effective component of galla chinensis as well as preparation method and use thereof

An effective component, gallnut technology, which can be used in drug combinations, medical raw materials derived from arthropods, anti-tumor drugs, etc., can solve problems such as side effects and involving normal cells.

Inactive Publication Date: 2012-04-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of galla chinensis as well as preparation method and use thereof
  • Effective component of galla chinensis as well as preparation method and use thereof
  • Effective component of galla chinensis as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 5

[0067] The preparation of embodiment 1 gallnut effective component

[0068] Crush 250g gallnut, add 8 times the amount of ethyl acetate: ethanol = 1:1, heat and reflux for 1 hour, extract twice, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extraction After concentrating the extract, dissolve the sample with 5% methanol, and pass it through the ODS-C18 column. First, use 5% ethanol as the mobile phase, and then replace it with 50% ethanol as the mobile phase to obtain the eluent, which is used to prepare the liquid phase Chromatography continues to separate the eluent, separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0069]

[0070] The flow rate is 10ml / min, the column temperature is room temperature; the sample is dissolved...

Embodiment 2 5

[0071] The analysis of embodiment 2 gallnut effective components

[0072] The HPLC-ELSD joint analysis to the Galla gall effective component of embodiment 1

[0073] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 70% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 30% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 50% 0.2% glacial acetic acid aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic ac...

Embodiment 3 5

[0076] Embodiment 3 Galla gall effective component preparation

[0077] Take any gallnut active ingredient in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, move the material to drip irrigation after the material is made, and drop the drug solution to 6-8°C liquid paraffin In the process of removing oil, 400 dropping pills were prepared.

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Abstract

The invention relates to an effective component of gallnut, which is characterized in that the effective component is obtained by the following: step one, using a mixture of ethyl acetate and ethanol as solvent to extract the gallnut; step two, using the ethanol to extract medicine dregs to obtain extract; step three, obtaining eluent through chromatography of the extract; and step four, using prepared liquid-phase chromatographic gradient to elute the eluent, and collecting the eluent for 16.0 to 20.0 minutes to obtain the effective component, wherein a mobile phase comprises water and methyl cyanide. The invention also discloses a preparation method for the effective component, a pharmaceutically acceptable preparation, and application thereof to preparing anti-tumor medicament.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Galla galla, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K35/64A61P35/00
Inventor贺庆刘雳程翼宇水文波葛志伟窦静霍阳
OwnerTIANJIN TASLY PHARMA CO LTD