A strain of Achromobacter xylosoxidans and use thereof for depredating clomazone
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A colorless bacillus, clomazone technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of insufficient understanding, high difficulty, secondary pollution, etc., and achieve the effect of efficient degradation
Inactive Publication Date: 2009-06-24
NORTHEAST AGRICULTURAL UNIVERSITY
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[0005] At present, the main ways to solve the harm of long-residue herbicides are: to develop new varieties of herbicides to replace the existing long-residue herbicides. However, due to insufficient understanding of the target enzymes, and the development of a new variety of pesticides requires hundreds of millions of dollars. Therefore, it is very difficult; the breeding of herbicide-resistant transgenic crop varieties requires a large amount of research funds and manpower, and it will go through a long research process, which is far away from practical application; using some chemical substances, namely herbicide antidotes ( Protective agent) removes the phytotoxicity caused by herbicides to crops, but involves the release of another chemical substance into the environment, which will cause secondary pollution; the use of microbial degradation from the soil can truly eliminate long residues Effective way of herbicide pollution
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Embodiment 1
[0019] Example 1. Isolation, purification and identification of Achromobacter xylosoxidans LYW CGMCC No. 2773
[0020] 1. Isolation and purification of Achromobacter xylosoxidans LYW CGMCC №.2773
[0021] 1) Collect samples
[0022] Soil samples were collected from the soil where the long-residual herbicide clomazone was applied for a long time in the experimental practice base of Northeast Agricultural University in Harbin, Heilongjiang Province.
[0023] 2) Enrichment and isolation of bacteria
[0024] Mix the soil samples of long-term application of long-residual herbicide evenly, weigh 10g and add to the solution containing 200mg·L -1 In 50mL enriched culture medium of clomazone, place at 30°C, 120r·min -1 shaker culture. Add 10ml of fresh enriched culture medium every week, increase the concentration of clomazone each time, so that the concentration is 200mg·L -1 , 400mg·L -1 , 800mg·L -1 , 1600mg·L -1 , 2000mg·L -1 , 3000mg·L -1 , 4000mg·L -1 , 5000mg·L -1 , ...
Embodiment 2
[0033] Example 2, Degradation of Clomazone in Soil by Achromobacter xylosoxidans LYW
[0034] 1. The content of Achromobacter xylosoxidans LYW at different temperatures is 750μg·kg -1 The degradation effect of clomazone in soil
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Abstract
The invention discloses an achromobacter xylosoxidans and the application thereof in the degradation of clomazone. The achromobacter xylosoxidans is achromobacter xylosoxidans CGMCC No. 2773. The bacterial strain can grow in the environment of high concentration clomazone, and can degrade the clomazone with high efficiency, so as to provide important foundation for the study of micro-biological degradation of residual weedicide clomazone, thus being applied to biological purification of the water body and soil which are polluted by the clomazone.
Description
technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and its application in degrading clomazone. Background technique [0002] Clomazone was first used to control weeds in soybean fields, and later it was widely used in various crop fields because of its outstanding characteristics of low dosage and high activity. Because it is highly safe for soybeans and has a wide herbicidal spectrum, especially effective against malignant weeds such as commelina, it is used in a large amount in Heilongjiang Province, with an annual amount of more than 300 tons, and there is an upward trend. However, the herbicide has a long duration of action, causing its biological activity in the soil to be as long as 6 months or more. Therefore, it is not suitable for planting wheat, barley, oats, rye, millet, wheat, barley, oats, Rice, sugar beet, rapeseed, etc., can be planted in the next year after use, such as rice, corn, cotton, peanut, sunflower, etc., seriousl...
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