Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ovary function associated protein, and preparation and use thereof

A technology related to protein and ovarian function, applied in the fields of botanical equipment and methods, biochemical equipment and methods, peptide/protein components, etc., which can solve problems such as imperfect theory and inability to prevent MPF activation.

Inactive Publication Date: 2009-07-15
ARMY MEDICAL UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is evidence that: (1) MAKP was not found to play a role in the activation of MPF in mos gene knockout mouse oocytes; (2) Inhibition of MAPK in rats could not prevent the activation of MPF, and meiosis could still be inhibited. (3) MPF activation independent of MAKP was found in the study of frog eggs
Therefore, the theory in this field is still quite incomplete and faces fierce debates. It is speculated that there may be unknown APC / C substrates in egg cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ovary function associated protein, and preparation and use thereof
  • Ovary function associated protein, and preparation and use thereof
  • Ovary function associated protein, and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The inventor first obtained the polypeptide fragment of MOP53 (the amino acid sequence is GDLVASFSLQDYNENPK) from the M II stage oocytes of pure-line BALB / C mice through two-dimensional electrophoresis (2-DE), MS analysis and computer bioinformatics analysis. , and then according to the amino acid sequence of the polypeptide fragment, adopt genetic engineering technology to clone the full-length cDNA sequence of MOP53 (as shown in SEQ ID No.1) and deduce the amino acid sequence of MOP53 (as shown in SEQ ID No.2) , and further expressed MOP53 by prokaryotic expression system and prepared polyclonal antibody against MOP53. Finally, the biological information, intracellular localization and tissue-specific distribution of MOP53 gene were extensively studied. The research results showed that: (1) MOP53 gene is specifically distributed in ovarian tissue; (2) MOP53 has special functional domains: WD40 and F-BOX, in which F-BOX has the ability to connect target proteins and ubi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an ovarian function-related protein. The ovarian function-related protein is protein consisting of amino acid sequences shown by SEQ ID No.2 or derived protein with the same or similar activity and formed by substituting / or deleting and / or adding the amino acid sequences shown by the SEQ ID No.2 by one or more amino acid sequences. The invention further discloses an encoding gene of the protein, a recombinant expression carrier containing the gene, a transformant containing the recombinant expression carrier, and a preparation method of the protein and an antibody thereof. The ovarian function-related protein possibly takes part in cell signal transduction and mature regulation of ova, plays a certain effect during a fertilization process, and shows potential of being further developed into a novel diagnostic reagent or a preventive drug for diseases caused by hypofunction, deletion or hyperfunction of the protein.

Description

technical field [0001] The invention relates to a protein, in particular to a protein related to ovarian function, and also relates to a preparation method and application of the protein. Background technique [0002] Oocytes (also known as eggs) are special, highly differentiated cells. It has organelles for its unique function and can go through a series of developmental stages until it acquires a cell type compatible with fertilization. During the human embryonic period, primordial germ cells (PGCs) undergo mitosis to complete the proliferation of oogonia, and the proliferated oogonia (called primary oocytes) enter the prophase of the first meiosis, and remain resting in this period after birth After that, under the stimulation of various signals from the undifferentiated cells of the gonad, the primary oocyte grows and differentiates until it matures into an egg cell. During this time, the egg cell needs to synthesize a large number of unique proteins in preparation fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/47C12N15/12C12N15/63C07K16/18G01N33/53C12Q1/68A61K38/17A61P15/08
Inventor 何畏吴玉章李彦锋龙玲向云龙
Owner ARMY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com