Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of Hirsutanols A in preparing anti-tumor medicine

An anti-tumor drug, tumor technology, applied in the direction of anti-tumor drugs, drug combination, drug delivery, etc., can solve the problem of unreported anti-tumor activity

Inactive Publication Date: 2011-09-21
SUN YAT SEN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antitumor activity of Hirsutanols A has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of Hirsutanols A in preparing anti-tumor medicine
  • Use of Hirsutanols A in preparing anti-tumor medicine
  • Use of Hirsutanols A in preparing anti-tumor medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of compound A

[0025] a. Seed culture of marine fungus Chondrostereum sp.nov.:

[0026] The strain uses PDA as the medium, and the composition is: 200g of potatoes, 20g of glucose, 20g of agar, and 1L of water. Make a test tube slant, pick the strains and insert them into the slant, culture at 25-28°C for 7 days, and then store at 4°C.

[0027] b. Fermentation culture of marine fungus Chondrostereum sp.nov.:

[0028] The composition of the fermentation medium is: 10g of glucose, 1g of yeast extract, 2g of peptone, 30g of coarse sea salt, and 1L of water; the strains cultivated in the slope are picked into the fermentation medium, and cultured at room temperature 25-28°C for 30 days;

[0029]c. Filtrating the above-mentioned cultivated fermentation broth, filtering out the bacteria, and collecting the culture fluid;

[0030] d. The culture solution was extracted 3 times with ethyl acetate with a volume ratio of 1:1, the extracts were com...

Embodiment 2

[0039] Embodiment 2: the antitumor activity test of compound A

[0040] a. Cell lines: nasopharyngeal carcinoma cell lines CNE2, SUNE1; breast cancer cell lines MCF-7, MDA-MB-453, MDA-MB-435; liver cancer cell line HEP3B.

[0041] b. Add the above-mentioned tumor cell lines with logarithmic growth into a 96-well plate, and each well contains 3000-8000 cells in 200 μL.

[0042] c. Then add the drug, set 7 drug concentration gradients, respectively 0, 0.39, 0.78, 1.56, 3.12, 6.25, 12.5, 25 μg / mL, and set a DMSO solvent control group.

[0043] d. Set up 4 parallel wells in each group and culture at 37°C for 72 hours. 10 μL of 5 mg / mL MTT solution was added 4 hours before the end of the experiment, cultured for another 4 hours, the culture solution was discarded, and 0.1 mL DMSO was added.

[0044] e. After the crystals are dissolved, detect the OD value of each well with dual wavelengths (570nm, 655nm) on an enzyme-linked detector. Calculate the growth inhibition rate accordin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of Hirsutanols A to preparation of an anti-tumor drug. A research finds that a compound, namely the Hirsutanols A shows powerful cytotoxicity to a plurality of tumor cell lines, for example, IC50 values to human nasopharyngeal carcinoma cell lines (CNE2 and SUNE1), human breast cancer cells (MCF-7, MDA-MB-435 and MDA-MB-453), and a human hepatocellular carcinoma cell (HEP3B) are 4.06, 3.44, 10.14, 9.86, 4.30 and 2.61mu g / mL respectively. The research shows that the Hirsutanols A can be used for preparing the anti-tumor drug, can be prepared into an anti-tumor drug composition with other drugs, and can be prepared with pharmaceutically acceptable accessories into dosage forms such as an injection, a tablet, a pill, a capsule, a solution, a suspension, anemulsion, and the like; and the administration route of the Hirsutanols A can be oral administration, vein administration, muscle administration or skin administration.

Description

technical field [0001] The present invention relates to the novel use of compound Hirsutanols A. Background technique [0002] Marine microorganisms are extremely rich in resources. Because the marine environment is very unique, such as: high salt, high pressure, low nutrition, no light, marine microorganisms have adapted to the unique living environment of the ocean, which must have created their unique metabolic pathways and genetic background. Studies have found that marine microorganisms can produce metabolites with unique chemical structures and various physiological activities, many of which cannot be produced by terrestrial microorganisms and are unique to marine microorganisms. Marine microorganisms can achieve large-scale fermentation and cultivation, and there is no resource problem in the preparation of active metabolites and drug development. Marine microorganisms have been considered the most important resource for discovering new marine drugs. At present, man...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61P35/00A61K9/48A61K9/10A61K9/107A61K9/08A61K9/20A61K31/122
Inventor 李厚金蓝文健朱孝峰邓蓉杨芬
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com