A kind of gerbera chrysanthemum tissue culture rapid propagation method
A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high cost, large differences in research results, high tissue culture costs, etc., and achieve tissue culture cost saving and reduction The transfer process and the effect of simplifying the tissue culture procedure
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Embodiment 1
[0013] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;
[0014] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 2mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA3.0mg / L and IAA0.1mg / L Among them, the number of inoculations per bottle is 25;
[0015] (3) The explants inoculated in the culture flask were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 800Lux, light time of 14h / d, and temperature of 28°C. In 65 days, the length of the induction obtained was 4cm adventitious buds, wherein the induction rate of the green pistil variety was 99.8%, and the induction rate of the black pistil variety was 91%;
[0016] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.3mg / L, the number of inoculations per bottle is 10, under the conditions of 1500Lux lig...
Embodiment 2
[0019] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;
[0020] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 4mm squares, and inoculate the leaves back down in MS medium supplemented with 6-BA 5.0 mg / L and IAA 0.3 mg / L , the number of inoculations per bottle is 10;
[0021] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under the conditions of light intensity of 1500Lux, light time of 13h / d, and temperature of 25°C under the conditions of green filter film. 50 days, the length that obtains induction is the adventitious bud of 8cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 97%;
[0022] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.2mg / L, the number of inoculations per bottle is 15, under the conditions of 800Lux ligh...
Embodiment 3
[0025] (1) cutting the gerbera test-tube plantlet leaves on the ultra-clean workbench as explants;
[0026] (2) Cut the explant 3 times along the vertical midrib direction, cut off the veins, cut into 6mm squares, and inoculate the leaves back down on MS medium supplemented with 6-BA6.0mg / L and IAA0.4mg / L Among them, the number of inoculations per bottle is 15;
[0027] (3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then placed under a green filter film under the conditions of light intensity of 2000Lux, light time of 12h / d, and temperature of 20°C. 50 days, the length that obtains induction is the adventitious bud of 7cm, wherein the induction rate of green pistil variety is 100%, and the induction rate of black pistil variety is 94%;
[0028] (4) Inoculate the obtained adventitious buds into the MS medium added with IAA0.1mg / L, the number of inoculations per bottle is 20, under the conditions of 2000Lux light intensi...
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