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Swine hepatitis E virus epitope and application thereof

A swine hepatitis E, virus antigen technology, applied in the directions of virus antigen components, virus peptides, antiviral agents, etc., can solve the problems of weak specificity and cannot specifically reflect the epidemic pattern of porcine hepatitis E.

Inactive Publication Date: 2011-09-14
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although porcine hepatitis E virus and human hepatitis E virus have a high sequence homology, there are still some differences between them. Using human hepatitis E virus epitopes to detect porcine hepatitis E virus leads to low detection specificity. Can not specifically reflect the prevalence of swine hepatitis E

Method used

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  • Swine hepatitis E virus epitope and application thereof
  • Swine hepatitis E virus epitope and application thereof
  • Swine hepatitis E virus epitope and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Porcine hepatitis E virus surface antigen

[0040] The computer software is used to screen the recognition epitopes from the corresponding proteins of the open reading frames ORF1, ORF2 and ORF3 of the whole genome of porcine hepatitis E virus. Epitope recognition is mainly based on the hydrophilicity, surface degree, flexibility of protein or polypeptide amino acid sequence, Chou-Fasman's helix, sheet and turn, Robson-Garnier's secondary structure and C- and N-terminus to predict Antigen comprehensive data (Antigenic Index, AI). Using existing software, such as: GCG (Madison, Wisconsin, USA) and DNAStar (Madison, Wisconsin, USA) to calculate AI and auxiliary recognition antigen regions from ORF1, ORF2 and ORF3 proteins of pig HEV. According to the amino acid sequences obtained from the known proteins of ORF1, ORF2 and ORF3 of porcine HEV, computer programs were used to estimate their antigenic regions (AI) (generally 10 to 15 amino acids).

[0041] After an...

Embodiment 2

[0106] Example 2 Screening of Porcine Hepatitis E Virus Epitopes

[0107] Firstly, the porcine HEV total antibody ELISA kit produced by Beijing Wantai Industrial Biomedicine Co., Ltd. was used to identify the collected porcine serum, and the porcine HEV positive serum and negative serum were screened out.

[0108] The porcine HEV polypeptide obtained in Example 1 was identified by ELISA with porcine HEV positive serum and negative serum, and porcine HEV epitope antigen was screened out. The specific steps are as follows:

[0109] 1. Antigen Dilution

[0110] Take 10 μl of the peptide with a concentration of 10 mg / ml (dissolved 5 mg of the synthesized peptide in 0.5 ml of dimethyl sulfoxide (DMSO)) and mix it with 90 ul of PBS to obtain an antigen dilution with a solubility of 1 μg / μl. Then take 15 μl of the diluted antigen solution and mix with 5 ml of the coating solution to obtain a diluted antigen solubility of 3 μg / ml. Add antigen dilution to 100 μl / well on the microtiter...

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Abstract

The invention provides a swine hepatitis E virus epitope expressed by SEQ ID No:1-8. The epitope of swine hepatitis E virus obtained by screening by the computer software and experimental verification, on one hand, is independently or compositely used in fast and special swine hepatitis E virus diagnostic reagent kit for fast diagnosing the swine hepatitis E virus, in particular for identifying diagnostic techniques of developing vaccination antibody and natural latent infection antibodies for monitoring the prevalence of the swine hepatitis E virus; on the other hand, for designing special, safe and efficient swine hepatitis E virus vaccine for preventing and controlling the prevalence of the swine hepatitis E virus and breaking the propagation from pig to people.

Description

technical field [0001] The invention relates to a hepatitis virus antigen epitope, in particular to a porcine hepatitis E virus antigen epitope and its application in vitro detection and vaccine. Background technique [0002] Hepatitis E virus (HEV) is a non-A-non-B hepatitis virus transmitted by fecal and oral routes. The virus can cause outbreaks in the population, mainly affecting young adults, and can cause a 20% fatality rate for pregnant women. From 1986 to 1988, Hepatitis E was prevalent in southern Xinjiang due to drinking water pollution, with a total of 119,280 cases and 707 deaths. It was the largest Hepatitis E epidemic in the world so far. In recent years, the incidence of hepatitis E has been increasing year by year. According to the statistics of the Ministry of Health of my country, the incidence of hepatitis E in 2003 increased by 40.4% compared with that in 2002. Therefore, the prevention and control of hepatitis E has been included in the work schedule o...

Claims

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Application Information

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IPC IPC(8): C07K14/08G01N33/576A61K39/12A61P31/14
Inventor 赵凯刘启文李震于瑞嵩唐雪明朱宏谭芙蓉吴萧王金斌
Owner SHANGHAI ACAD OF AGRI SCI
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