Seed breeding and application of rape environmental sensitivity nucleus sterile line with self-incompatibility characteristic
An environment-sensitive, nuclear sterile line technology, applied in the field of selection and application of rapeseed male sterile lines, environment-sensitive nuclear sterile lines of rapeseed, can solve the problems of large seed production failure, unguaranteed seed purity, and poor fertility. Instability and other problems, to achieve the effect of improving purity and reducing the probability of self-fertilization
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Embodiment 1
[0039] This example can illustrate the breeding process of the self-incompatibility+environment-sensitive GMS line.
[0040] Zhong2SI with self-incompatibility (Brassica napus "Zhongshuang 2" crossed with self-incompatibility Brassica napus) was crossed with environmental sensitive genic male sterile line Z2EGMS (Z2EGMS line It was bred by crossing the environmentally sensitive sterile strain Q8S found in Qinyou 8 as the male parent and Zhongshuang 2). get F 1 Generation selfing, get F 2 , F 2 Environmentally sensitive sterile plants appeared in the generation, and they were sprayed with 10% saline to make them self-fertile.
[0041] The offspring are planted according to the line, so that they are in a greenhouse environment with a temperature of 20 / 10°C (day / night) and a photoperiod of 11h / 13h (day / night) from the budding stage to the flowering stage, and some inflorescences of the plants are selected for self-bagging; the other part The inflorescences are bagged, and th...
Embodiment 2
[0043] In this example, the fertility identification of the self-incompatibility+environment-sensitive GMS line was carried out.
[0044] If Z2SIS is placed in a greenhouse environment with a temperature of 26°C / day, 16°C / night, and a photoperiod of 14h / day, 10h / night, it will be completely sterile and unable to self-fertilize. In a greenhouse environment with a temperature of 20°C / day, 10°C / night, and a photoperiod of 10h / day, 14h / night, it is highly fertile, but self-fertilization cannot be fruitful, and it can be fruitful after spraying 10% saline water. 4.8 grains / pod.
[0045] Z2SIS and control Z2EGMS were sown in autumn in Datian, Yangling, Shaanxi, and the fertility identification was carried out in the next year. Due to the low temperature and short photoperiod in March, the temperature rose rapidly in April and the photoperiod became longer. The two sterile lines were partially fertile for 7-10 days in early April, and then turned sterile. The self-pollination of th...
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