Seed selection and application of rape environmental sensitivity nuclear sterile line with self-incompatibility characteristic

An environment-sensitive, nuclear sterile line technology, applied to the breeding and application of rapeseed environmentally sensitive nuclear sterile lines, the field of rapeseed male sterile lines, can solve the problem that the purity of seeds cannot be guaranteed, the failure of seed production is large, and the promotion is limited. and other problems to achieve the effect of reducing the probability of self-fertilization and improving the purity

Inactive Publication Date: 2009-12-09
NORTHWEST A & F UNIV
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest disadvantage of environmentally sensitive sterile lines is that their fertility is not stable. In the seed production area, if the flowering period encounters special weather such as continuous rain and cooling, the fertility will be restored temporarily (1 to 3 days), and self-fertilizing seeds will be mixed into hybrids. Seed purity cannot be guaranteed, and the risk of seed production failure is relatively high, which limits the promotion of these two-line hybrid rapeseed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This example can illustrate the breeding process of the self-incompatibility+environment-sensitive GMS line.

[0040] Zhong2 SI with self-incompatibility (Brassica napus "Zhongshuang 2" crossed with self-incompatibility Brassica napus) was crossed with environmental sensitive genic male sterile line Z2EGMS ( The Z2EGMS line was bred by crossing the environmentally sensitive sterile strain Q8S found in Qinyou 8 as the male parent and Zhongshuang 2). get F 1 Generation selfing, get F 2 , F 2 Environmentally sensitive sterile plants appeared in the generation, and they were sprayed with 10% saline to make them self-fertile.

[0041] The offspring are planted according to the line, so that they are in a greenhouse environment with a temperature of 20 / 10°C (day / night) and a photoperiod of 11h / 13h (day / night) from the budding stage to the flowering stage, and some inflorescences of the plants are selected for self-bagging; the other part The inflorescences are bagged, and...

Embodiment 2

[0043] In this example, the fertility identification of the self-incompatibility+environment-sensitive GMS line was carried out.

[0044] If the Z2 SIS is placed in a greenhouse environment with a temperature of 26°C / day, 16°C / night, and a photoperiod of 14h / day, 10h / night, it will be completely sterile and unable to self-fertilize. In a greenhouse environment with a temperature of 20°C / day, 10°C / night, and a photoperiod of 10h / day, 14h / night, it is highly fertile, but self-fertilization cannot be fruitful, and it can be fruitful after spraying 10% saline water. 4.8 grains / pod.

[0045] Z2 SIS and control Z2 EGMS were sown in autumn in Datian, Yangling, Shaanxi, and the fertility identification was carried out in the next year. Due to the low temperature and short photoperiod in March, the temperature rose rapidly in April and the photoperiod became longer. The two sterile lines were partially fertile for 7-10 days in early April, and then turned sterile. The self-pollinatio...

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PUM

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Abstract

The invention discloses a seed selection method of rape environmental sensitivity nuclear sterile line with self-incompatibility characteristic. In the method, nuclear sterile rape sensitive to photoperiod, temperature and the like is selected to hybridize with self-incompatibility line, environmental sensitivity nuclear sterile line with self-incompatibility characteristic is selected from the progeny as a female parent of two-line hybrid cultivar. The rape environmental sensitivity nuclear sterile line with self-incompatibility characteristic selected by the invention is applied to production of hybrid seeds, selfing fructification probability of the environmental sensitivity sterile line in the production link can be reduced and purity of the hybrid seeds can be improved.

Description

technical field [0001] The invention belongs to the field of obtaining new plants, and relates to a rapeseed male sterile line, in particular to the selection and application of a rapeseed environment-sensitive male sterile line with self-incompatibility characteristics. Background technique [0002] The utilization of crop heterosis must rely on a stable and reliable pollination control system. Self-incompatibility and environment-sensitive nuclear male sterility are two important pollination control systems in rapeseed. Self-incompatibility phenomenon widely exists in Brassica napus x Brassica napus hybrid progenies, Chinese cabbage and Brassica napus (Yang Guangsheng, Fu Tingdong, Self-incompatibility hybrids of rapeseed, edited by Fu Tingdong: Breeding and Utilization (Second Edition), Hubei Science and Technology Press, 2000, p112-148). The self-compatibility index of strict self-incompatibility lines (dividing artificially pollinated self-fertilized seeds by the numb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02
Inventor 于澄宇胡胜武
Owner NORTHWEST A & F UNIV
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