HCV genotyping DNA microarray chip

A microarray chip, genotyping technology, applied in DNA/RNA fragmentation, recombinant DNA technology, microorganism determination/inspection, etc., can solve the problems of cumbersome operation, low throughput, low sensitivity, etc.
CN101660002AInactive Publication Date: 2010-03-03DAAN GENE CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DAAN GENE CO LTD
Publication Date
2010-03-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a DNA microarray chip, in particular to a hepatitis C virus (HCV) genotyping DNA microarray chip. A solid-phase carrier substrate is used, and an oligonucleotide probe is designed in view of different genotypes of HCV so as to prepare the DNA microarray chip; and when matched with a PCR primer and other components, the DNA microarray chip can quickly and accurately classify the types of the hepatitis C virus in a blood sample.
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Description

Technical field

[0001] The invention relates to a DNA microarray chip, in particular to a hepatitis C virus (HCV) genotyping DNA microarray chip. The invention adopts a solid-phase carrier substrate, designs oligonucleotide probes according to different genotypes of HCV, and prepares a DNA microarray chip for rapid and accurate typing detection of hepatitis C virus in blood samples. Background technique

[0002] Hepatitis C is an infectious disease that seriously harms human health and has a wide range of epidemics. Its pathogen is hepatitis C virus (HCV). At present, there are more than 170 million people infected with HCV in the world, and more than 100,000 HCV patients develop liver cancer each year, and then have gastrointestinal bleeding and ascites. According to the WHO 2002 annual report, in 2001, chronic liver disease caused 14 million deaths, including 796,000 cases caused by cirrhosis and 616,000 cases caused by primary liver cancer.

[0003] HCV is a positive single-st...

Claims

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