Method for extracting and detecting hydrocortisone in hairs

A hydrocortisone and detection method technology, which is applied in the field of clinical medical analysis, can solve the problems that are difficult to popularize and have not been applied to the detection of hair

Inactive Publication Date: 2012-04-11
SOUTHEAST UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although LC-MS is accurate and sensitive, it requires expensive instruments, so it is difficult to popularize; although HPLC method does not require complex and expensive instruments and has high sensitivity, it is still only used in the determination of blood, urine and saliva, and has not been applied to the detection Report of hydrocortisone in hair

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and detecting hydrocortisone in hairs
  • Method for extracting and detecting hydrocortisone in hairs

Examples

Experimental program
Comparison scheme
Effect test

example

[0054] Detection of hydrocortisone in the hair of 32 Han normal people:

[0055] 1. Extraction of hydrocortisone in hair:

[0056] About 100 mg of hair was taken each time, washed twice with 5 ml of methanol at room temperature (2 min each time), and then ground with a ball mill. Weigh 50mg of hair powder, add 1ml of 0.1M HCl, and incubate at 40°C for 16h. After incubation, the incubation solution was centrifuged, the supernatant was taken, and hydrocortisone was extracted with ethyl acetate, and the ethyl acetate layer was washed with pure N 2 blow dry.

[0057] 2. Derivatization, separation and fluorescence detection of hydrocortisone in hair

[0058] The dried material was derivatized with 50 μL of sulfuric acid / ethanol solution (volume ratio 70 / 30) at 70° C. for 2 minutes, then 500 μL of water was added to stop the derivatization reaction, and placed in a refrigerator. After freezing for 10 minutes, take out the derivative substance, add 50 μL of 1000ng / ml fluorescein ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting and detecting hydrocortisone in hairs, which can be used for extracting and detecting the hydrocortisone in the hairs. In a labeled recovery experiment, the recovery rate is 102.5+ / -13.0 percent, the extraction recovery rate is 103.3+ / -5.2 percent, the intra-day standard deviation is less than 7.3 percent, and the inter-day standard deviation is less than 8.5 percent. The labeled recovery experiment proves that the detection technique is accurate and reliable. The method comprises the following steps of: a, extracting the hydrocortisone in the hairs; b, detecting the hydrocortisone in the hairs; and c, detecting the chromatogram of the hydrocortisone by using a fluorescence detector.

Description

technical field [0001] The invention is a new technology for extracting and detecting hydrocortisone in hair, and belongs to the technical field of clinical medical analysis. Background technique [0002] The adrenal cortex hormone hydrocortisone is an essential hormone for maintaining life, and its content in the body has varying degrees of influence on the functions of substance metabolism, vascular response, nervous system, urinary system and digestive system; hydrocortisone reflects the hypothalamic - The activity of the pituitary-adrenal axis (HPA). If the level of adrenal cortex hormones is low, the human body's ability to adapt to external damaging stimuli or severe environmental changes will be greatly reduced. In addition, hydrocortisone is a very important adrenocortical hormone, an important biological indicator of psychological stress response, and has become an important indicator for evaluating psychological stress. Therefore, the level of hydrocortisone is an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N1/28G01N30/74
Inventor 邓慧华皋伟陆祖宏谢巧珍
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap