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Eu-MOF nano fluorescent probe, preparation method thereof and application of Eu-MOF nano fluorescent probe in detection of anthrax biomarkers

A nano-fluorescent probe and biomarker technology, applied in the field of nano-materials, can solve problems such as difficulty in satisfying online detection, difficulty in achieving obvious color changes, and high cost of detection technology, and achieve good specificity, specific visual detection, The effect of promoting the antenna effect

Inactive Publication Date: 2021-08-27
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection technology is expensive to detect, requires complex sample processing technology, professional operators, and it is difficult to achieve obvious color changes visible to the naked eye, and it is difficult to meet the requirements of online detection

Method used

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  • Eu-MOF nano fluorescent probe, preparation method thereof and application of Eu-MOF nano fluorescent probe in detection of anthrax biomarkers
  • Eu-MOF nano fluorescent probe, preparation method thereof and application of Eu-MOF nano fluorescent probe in detection of anthrax biomarkers
  • Eu-MOF nano fluorescent probe, preparation method thereof and application of Eu-MOF nano fluorescent probe in detection of anthrax biomarkers

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Experimental program
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Effect test

Embodiment 1

[0024] The preparation of embodiment 1Eu-MOF nano fluorescent probe

[0025] 1. Preparation of Eu-MOF Nanofluorescent Probes

[0026] 0.1351g Al(NO 3 ) 3 9H 2 O, 0.1196 g of terephthalic acid was dissolved in a beaker with 18 mL of DMF, then 18 mL of water and ethanol were added with constant stirring. After continuous stirring for 10 minutes, the mixed solution was transferred to a polytetrafluoroethylene reactor and reacted in an oven at 145° C. for 9 hours. Finally, the suspension was centrifuged, washed several times with distilled water and absolute ethanol, and dried in a vacuum oven at 70 °C to obtain the white powder product AM.

[0027] 400mg Eu(NO 3 ) 3 ·6H 2 O was added to a 50 mL round bottom flask containing 20 mL of anhydrous methanol and dissolved by sonication for 5 min. 200 mg AM was then added to the flask and dissolved by sonication for 10 minutes. After magnetic stirring at room temperature for 12 hours, anhydrous methanol was added for centrifugat...

Embodiment 2E

[0034] Example 2 Application of Eu-MOF Nano-fluorescent Probes in Detection of Anthrax Biomarkers

[0035] 100mg Eu 3+ @AM was dissolved in 100 mL pH=7.10 Tris-HCl (0.05 mol / L), sonicated for 10 minutes, and stored for later use. Then, the above-prepared Eu 3+ @AM 1000 μL, 300 μL of DPA standard solutions of different concentrations and 1700 μL of Tris-HCl (0.05 mol / L) at pH = 7.10 were sequentially added to the centrifuge tube with a stopper. After mixing for 5 minutes, the fluorescence spectrum was measured at an excitation wavelength of 280 nm.

[0036] Figure 6 Schematic diagram of the detection mechanism for the Eu@AM fluorescent probe to detect DPA. DPA can work with Eu 3+ coordinate to promote the antenna effect, thereby effectively sensitizing Eu 3+ The characteristic red emission (614nm) of , so that the naked eye can identify Eu 3+ @AM Fluorescent change from blue to red. Before adding DPA, terephthalic acid absorbs UV light and transfers energy to Al 3+ , ...

Embodiment 3

[0037] Example 3 Interference of common ions on the detection of DPA by Eu-MOF nanofluorescent probes

[0038]The test of interference experiment is according to the fluorescence condition described in embodiment 2, and common ion (K + , Ca 2+ , Na + , Mg 2+ , Fe 2+ , Cl - , NO 3 - and CO 3 2- ), amino acids (L-phenylalanine, D-aspartic acid, glutamine, methionine, L-cysteine, and glycine (L-Phe, Asp, Glu, Met, L-Cys, and Gly)) The concentrations of aromatic compounds (benzoic acid, phthalic acid, isophthalic acid (BA, o-PA and m-PA)) were 200 μmol / L, and the concentration of target DPA was 2 μmol / L.

[0039] Figure 5 For the detection effect of Eu@AM fluorescent probe on DPA in the presence of common ions. Add common ions (K + , Ca 2+ , Na + , Mg 2+ , Fe 2+ , Cl - , NO 3 - and CO 3 2- ), amino acids (L-phenylalanine, D-aspartic acid, glutamine, methionine, L-cysteine, and glycine (L-Phe, Asp, Glu, Met, L-Cys, and Gly)) and the relative fluorescence inte...

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Abstract

The invention relates to the technical field of nano materials, in particular to an Eu-MOF nano fluorescent probe. A preparation method comprises the following steps of: dissolving Al (NO3)3.9H2O and terephthalic acid in a container filled with DMF (Dimethyl Formamide), adding water and ethanol under a stirring condition, continuing to stir, transferring the obtained solution into a polytetrafluoroethylene reactor for reaction, and centrifuging, washing and drying to obtain AM (Acrylamide) after the reaction is finished; adding Eu (NO3)3.6H2O and absolute methanol into a container, ultrasonically dissolving the added substances, adding and ultrasonically dissolving the AM, stirring, centrifugally separating, washing, drying and grinding to obtain a target product Eu < 3 + >@AM. The detection technology designed by the invention has the advantages of simplicity, low cost, rapidness, accuracy, sensitivity, good specificity and visual detection.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a Eu-MOF nanometer fluorescent probe, a preparation method thereof and an application in detecting anthrax biomarkers. Background technique [0002] Anthrax is caused by Bacillus anthracis, which is a zoonotic acute infectious disease that seriously threatens human life and health. Therefore, accurate, rapid and sensitive detection of dipicolinic acid (DPA), a biomarker of anthrax spores, is crucial for the prevention and control of disease outbreaks. Traditional detection technology is expensive to detect, requires complex sample processing technology, professional operators, and it is difficult to achieve obvious color changes visible to the naked eye, and it is difficult to meet the requirements of online detection. Contents of the invention [0003] In view of the above problems, the present invention provides a Eu-MOF nano fluorescent probe, its preparation method a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 张蕾伊魁宇
Owner LIAONING UNIVERSITY
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