Detection method for acetylcholine in royal jelly

A technology of acetylcholine and detection methods, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc.

Inactive Publication Date: 2012-04-25
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, to the best of our knowledge, neither of these analytical techniques has been applied to the detection of choline or acetylcholine

Method used

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  • Detection method for acetylcholine in royal jelly
  • Detection method for acetylcholine in royal jelly

Examples

Experimental program
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Embodiment Construction

[0013] 1 Reagents and instruments

[0014] Acetylcholine standard; royal jelly; oven; capillary electrophoresis; electrochemiluminescence.

[0015] 2 methods

[0016] 1) Pretreatment of royal jelly:

[0017] Acetylcholine is a quaternary ammonium base, which itself cannot enhance or weaken the electrochemiluminescent signal of ruthenium bipyridyl. In this technology, the royal jelly is baked in an oven at 100°C for 10 hours to completely volatilize the water, then ground, weigh about 1g of the powder and put it in a closed container (the container is decompressed first), and then put it in Bake in an oven at 150°C for 3 minutes. During this process, acetylcholine undergoes a decomposition reaction to produce trimethylamine, which is easily soluble in water. Immediately use a syringe to inject 3mL of water into a closed container to absorb trimethylamine, and the obtained solution can be used after centrifugation and filtration.

[0018] 2) Capillary electrophoresis separa...

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Abstract

The invention provides a method for detecting acetylcholine in royal jelly, which is established by combining capillary electrophoresis separation with an electrochemiluminescence detection technique. The method comprises the following: a, a step of pretreating royal jelly; b, a step of performing capillary electrophoresis separation; and c, a step of performing electrochemiluminescence detection, wherein a target component separated out through capillary electrophoresis flows into an electrochemiluminescence detection tank so as to be detected; a selected detection system is a classical tris(bipyridine) ruthenium detection system which contains 2 to 8 mM of ruthenium solution, has the pH approximately between 7.0 and 9.0, adopts a three-electrode system, takes a platinum wire as a counter electrode, takes a silver-silver chloride electrode as a reference electrode, and takes 500-mu m platinum disk electrode as a working electrode; the peaking time of the target component and an interfering component differs by 200 to 250 second; and the quantitative detection of the acetylcholine in a royal jelly sample can be performed by a standard curve method. The method has the advantages of simplicity, economical efficiency and good accuracy.

Description

technical field [0001] The invention is a new technology for detecting acetylcholine in royal jelly, and belongs to the technical field of acetylcholine content detection. Background technique [0002] There is a large amount of acetylcholine in human brain tissue, but the content of acetylcholine will decrease with age, causing various mental diseases related to memory, such as Alzheimer's disease. Maintaining and increasing the content of acetylcholine in the brain is the fundamental way to solve memory loss. Acetylcholine mostly exists in the form of choline in nature. Choline must have a biochemical reaction with acetyl-CoA in the human body to synthesize acetylcholine with physiological activity. Royal jelly exists directly in the form of acetylcholine, which can be directly absorbed and utilized by the human body. Therefore, royal jelly has a certain effect on the prevention of Alzheimer's disease and memory loss. Therefore, the detection of acetylcholine in royal je...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N21/66
Inventor 卫伟康学军邓慧华陆祖宏
Owner SOUTHEAST UNIV
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