Novel method for crop cell breeding

A new method and crop technology, applied in the field of plant genetics and breeding, can solve the problems of poor agronomic traits, poor quality traits, and difficulty in breeding new wheat varieties, and achieve the effects of high cultivating power and improving crop breeding efficiency.

Active Publication Date: 2010-06-23
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

But at the same time, it was found that the main agronomic and quality traits of some existing high-cultivation genotypes are poor, and it is difficult to directly apply them to the breeding of new wheat varieties.

Method used

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  • Novel method for crop cell breeding
  • Novel method for crop cell breeding
  • Novel method for crop cell breeding

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. Creation and screening of materials with high anther cultivability in wheat

[0024] 1. Experimental materials

[0025] 92 wheat genotypes mainly including the main varieties for current agricultural production and high-generation stable lines. (The above-mentioned materials are preserved in the laboratory of the applicant and can be distributed to the public free of charge.)

[0026] 2. Collection period

[0027] Wheat pollen develops to uninucleated mid-late stage.

[0028] 3. Experimental method

[0029] (1) Young ears of wheat at the right stage were selected in the field, wrapped in wet gauze and placed in a 4°C refrigerator for pretreatment for 24-48 hours.

[0030] (2) Peel off the young ears of wheat and use 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterile distilled water 3-4 times, take out the anthers one by one with tweezers and place them on the induction medium (see attached table 1), about 100 anthers are placed in each bottle of medium.

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Abstract

The invention relates to a novel method for crop cell breeding, which relates to a novel variety breeding technique of the field of the plant genetics breeding. The novel method for crop cell breeding, which is provided by the invention, comprises the following steps: (1) selecting a plurality of crop genotypes to carry out anther culture in vitro, and screening out a crop backbone parent strain with high culture ability; (2) carrying out hybridization on the crop backbone parent strain and a main cultivated variety, and adopting anther culture in vitro to obtain a novel crop strain of a dynamic backbone parent strain cluster; and (3) carrying out hybridization, mutagenic treatment and composite hybridization accumulation on the crop backbone parent strain with high culture ability and the novel crop strain of the dynamic backbone parent strain cluster. The crop is a gramineous plant or a cruciferous plant. The method of the invention utilizes the dynamic backbone parent strain cluster with high culture ability to carry out composite hybridization and anther culture, realizes rapid integration of the genes with high yield, excellent quality and multiple resistances, and greatly improves the crop breeding efficiency. The technology is suitable for other crops, such as paddy rice, wheat, barley, corns, rapes, Chinese cabbages and the like, and the cruciferous plants.

Description

Technical field: [0001] The invention relates to a new method for improving crop cell breeding efficiency, which belongs to the field of plant genetic breeding. Background technique: [0002] Wheat is the second major food crop in my country, and wheat production accounts for about 22% of my country's grain production. However, the current wheat varieties promoted in production cannot meet the needs of domestic and foreign markets in terms of quality, high yield, and disease resistance. It is of great significance to strengthen the breeding of new wheat varieties with high quality, high yield, and multi-resistance. Although conventional breeding methods still play an important role in the current selection of new varieties, due to the limitations of traditional breeding methods such as long breeding cycle, lack of resources, and low selection efficiency, it has been difficult to support the development of modern agriculture for the cultivation of major new varieties of wheat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/06A01H4/00
Inventor 刘录祥赵林姝郭会君赵世荣古佳玉李军辉
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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