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Disposable polymerase chain reaction (pcr) chip and device

A technology of chain reaction and polymerase, applied in enzyme production/bioreactor, chemical instrument and method, biochemical equipment and method, etc.

Inactive Publication Date: 2010-06-23
UNIVERSITI SAINS MALAYSIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0018] However, due to miniaturization, the above-mentioned ratio of surface area to liquid volume increases, which may lead to the non-specific adsorption of biological samples to the channel surface and its viscoelastic flow characteristics may become obvious, which may be limited in PCR Amplification in Microfluidic Devices

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  • Disposable polymerase chain reaction (pcr) chip and device
  • Disposable polymerase chain reaction (pcr) chip and device
  • Disposable polymerase chain reaction (pcr) chip and device

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Embodiment Construction

[0029] The present invention relates to a polymerase chain reaction disc (PCRDisc) which takes advantage of static sample chambers and continuous flow PCR devices. Hereinafter, this specification will describe the present invention according to preferred embodiments of the present invention. However, it should be understood that this specification describes the present invention with preferred embodiments only for easier understanding of the present invention. Therefore, it is foreseeable that those skilled in the art to which the present invention pertains can make various modifications and equivalent designs to the present invention without departing from the scope described in the claims.

[0030] The following detailed description will be described with reference to any single or combined figures.

[0031] The invention relates to a disposable PCR device which includes a sample chamber, and the sample chamber is driven by a rotary-linear operation system to move from one ...

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Abstract

The present invention provides a polymerase chain reaction (PCR) device which comprises the following components: a chip kit 2, a plurality of sample chambers 1 provided in the chip kit for carrying the sample, and a heating device, wherein the chip kit is placed on the heating device. The chip kit can perform effective rotation on the heating device. A rotating wheel assists the rotation of the chip, wherein the heating device comprises a plurality of temperature bands which are set the sample chamber can move from one temperature band to another temperature band with a rotation-linear movement system when the chip rotates.

Description

technical field [0001] The present invention relates to multiplex polymerase chain reaction (PCR) devices. In particular, the present invention relates to a disposable PCR device, wherein the device includes a sample chamber, and the sample chamber is driven from one temperature to another temperature zone by a rotary-linear operation system. Background technique [0002] Polymerase chain reaction (PCR) is an important method capable of amplifying specific deoxyribonucleic acid (DNA) by a factor of up to 230 without simultaneously amplifying other genetic material in solution . This technique was first introduced in 1986, which is a major breakthrough in the application of molecular biology. The amplification method described mimics the natural DNA replication and repair programs and protein expression commonly found in natural biochemical processes. [0003] The replication of DNA from a single strand of DNA is carried out by specific enzymes, such as DNA polymerase. Sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40
CPCG01N2035/00158B01L7/525B01L2300/1805G01N2035/0439G01N35/025C12Q1/686
Inventor A·司马义S·达马林加姆L·江
Owner UNIVERSITI SAINS MALAYSIA