Medicine composition for treating cardio-cerebral-vascular diseases and preparation method and application thereof

Abstract

The invention discloses a medicine composition for treating cardio-cerebral-vascular diseases and a preparation method and application thereof, belonging to the field of traditional Chinese medicines. The effective component of the medicine composition comprises total sapogenin and total alkaloid extracted from traditional Chinese herb trichosanthes kirilowii Maxim and allium macrostemon. The medicine composition which can be made into proper preparation forms has good treatment effects on coronary disease, angina, arteriosclerosis, chest pain, hyperlipoidemia and the like. The invention also discloses preparation methods of the total sapogenin and the total alkaloid.

Description

technical field

[0001] The invention relates to a pharmaceutical composition for treating cardiovascular diseases, in particular to a pharmaceutical composition with extracts of traditional Chinese medicine Trichosanthes mellifera and Xiebai as active ingredients, a preparation method and application thereof. It belongs to the field of traditional Chinese medicine. Background technique

[0002] Cardiovascular disease, cerebrovascular disease, and malignant tumor are known as the three major human death diseases, and their prevention and in-depth research have always been the focus in the field of medicine. Cardiovascular diseases are currently common coronary heart disease, hypertension, angina pectoris, acute myocardial infarction, hyperlipidemia, etc., with a wide range of incidence and a greater risk of life-threatening. At present, traditional western medicine and traditional Chinese medicine have limitations in the treatment of cardiovascular diseases. For example, wes...

Examples

Embodiment 1

[0138] Example 1: Preparation of Total Alkaloids of Fructus Trichosanthis Fructus

[0139] Take 5 kg of Trichosanthes and 5 kg of Allium, add 40% ethanol to reflux and extract twice, each time for 1 hour, combine the filtrates, recover the ethanol under reduced pressure and concentrate it to 60 ° C to measure the relative density to 1.10, let it cool, add ethanol to make the alcohol content reach 70%, stand overnight, filter, the filtrate recovers ethanol under reduced pressure, concentrates appropriately, adds hydrochloric acid to adjust pH2, passes through a cation exchange resin column, first elutes with water, and the water washing liquid is for later use; then elutes with 5% sodium chloride solution to If there is no alkaloid reaction, the sodium chloride eluate is collected, concentrated and dried, and extracted three times with absolute ethanol under reflux, combined, the ethanol is recovered under reduced pressure, concentrated, and dried to obtain the total alkaloids (...

Embodiment 2

[0140] Example 2: Preparation of Total Alkaloids of Fructus Trichosanthis

[0141] Take 1.5 kg of Trichosanthes meloni and 1 kg of Allium scallops, add 30% ethanol to extract 3 times, each time for 0.5 hours, combine the filtrates, recover the ethanol under reduced pressure and concentrate to 60 ° C to measure the relative density to 1.05, let it cool, add ethanol to make the alcohol content reach 50%, left overnight, filtered, the filtrate was decompressed to recover ethanol, concentrated appropriately, added hydrochloric acid to adjust the pH to 1, passed through a cation exchange resin column, first eluted with water, and the water washing solution was used for later use; then eluted with 20% sodium chloride solution to If there is no alkaloid reaction, the sodium chloride eluate is collected, concentrated and dried, extracted twice with absolute ethanol under reflux, combined, the ethanol is recovered under reduced pressure, concentrated, and dried to obtain the total alkal...

Embodiment 3

[0142] Example 3: Preparation of Total Alkaloids of Fructus Trichosanthis

[0143] Take 6 kg of Trichosanthes and 2 kg of Scallops, add 60% ethanol to reflux and extract once for 3 hours each time, combine the filtrates, recover the ethanol under reduced pressure and concentrate to 60 ° C to measure the relative density to 1.25, let it cool, add ethanol to make the alcohol content reach 80%, stand overnight, filter, the filtrate recovers ethanol under reduced pressure, concentrates appropriately, adds hydrochloric acid to adjust the pH to 4, passes through a cation exchange resin column, first elutes with water, and the water washing solution is used for later use; then elutes with 2% sodium acetate solution until nothing For alkaloid reaction, the sodium acetate eluate was collected, concentrated and dried, refluxed with absolute ethanol to extract 3 times, combined, the ethanol was recovered under reduced pressure, concentrated, and dried to obtain the total alkaloids (total ...

technical field

[0001] The invention relates to a pharmaceutical composition for treating cardiovascular diseases, in particular to a pharmaceutical composition with extracts of traditional Chinese medicine Trichosanthes mellifera and Xiebai as active ingredients, a preparation method and application thereof. It belongs to the field of traditional Chinese medicine. Background technique

[0002] Cardiovascular disease, cerebrovascular disease, and malignant tumor are known as the three major human death diseases, and their prevention and in-depth research have always been the focus in the field of medicine. Cardiovascular diseases are currently common coronary heart disease, hypertension, angina pectoris, acute myocardial infarction, hyperlipidemia, etc., with a wide range of incidence and a greater risk of life-threatening. At present, traditional western medicine and traditional Chinese medicine have limitations in the treatment of cardiovascular diseases. For example, wes...

Examples

Embodiment 1

[0138] Example 1: Preparation of Total Alkaloids of Fructus Trichosanthis Fructus

[0139] Take 5 kg of Trichosanthes and 5 kg of Allium, add 40% ethanol to reflux and extract twice, each time for 1 hour, combine the filtrates, recover the ethanol under reduced pressure and concentrate it to 60 ° C to measure the relative density to 1.10, let it cool, add ethanol to make the alcohol content reach 70%, stand overnight, filter, the filtrate recovers ethanol under reduced pressure, concentrates appropriately, adds hydrochloric acid to adjust pH2, passes through a cation exchange resin column, first elutes with water, and the water washing liquid is for later use; then elutes with 5% sodium chloride solution to If there is no alkaloid reaction, the sodium chloride eluate is collected, concentrated and dried, and extracted three times with absolute ethanol under reflux, combined, the ethanol is recovered under reduced pressure, concentrated, and dried to obtain the total alkaloids (...

Embodiment 2

[0140] Example 2: Preparation of Total Alkaloids of Fructus Trichosanthis

[0141] Take 1.5 kg of Trichosanthes meloni and 1 kg of Allium scallops, add 30% ethanol to extract 3 times, each time for 0.5 hours, combine the filtrates, recover the ethanol under reduced pressure and concentrate to 60 ° C to measure the relative density to 1.05, let it cool, add ethanol to make the alcohol content reach 50%, left overnight, filtered, the filtrate was decompressed to recover ethanol, concentrated appropriately, added hydrochloric acid to adjust the pH to 1, passed through a cation exchange resin column, first eluted with water, and the water washing solution was used for later use; then eluted with 20% sodium chloride solution to If there is no alkaloid reaction, the sodium chloride eluate is collected, concentrated and dried, extracted twice with absolute ethanol under reflux, combined, the ethanol is recovered under reduced pressure, concentrated, and dried to obtain the total alkal...

Embodiment 3

[0142] Example 3: Preparation of Total Alkaloids of Fructus Trichosanthis

[0143] Take 6 kg of Trichosanthes and 2 kg of Scallops, add 60% ethanol to reflux and extract once for 3 hours each time, combine the filtrates, recover the ethanol under reduced pressure and concentrate to 60 ° C to measure the relative density to 1.25, let it cool, add ethanol to make the alcohol content reach 80%, stand overnight, filter, the filtrate recovers ethanol under reduced pressure, concentrates appropriately, adds hydrochloric acid to adjust the pH to 4, passes through a cation exchange resin column, first elutes with water, and the water washing solution is used for later use; then elutes with 2% sodium acetate solution until nothing For alkaloid reaction, the sodium acetate eluate was collected, concentrated and dried, refluxed with absolute ethanol to extract 3 times, combined, the ethanol was recovered under reduced pressure, concentrated, and dried to obtain the total alkaloids (total ...

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