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Auxiliary method for identifying wheat with different thousand grain weight properties, and special primer thereof

A technology of auxiliary identification and thousand-grain weight, applied in the direction of botanical equipment and methods, biochemical equipment and methods, applications, etc., to achieve the effects of simple operation, accelerated breeding speed, and reduced breeding costs

Inactive Publication Date: 2010-07-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the cloning of the GIF gene in wheat

Method used

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  • Auxiliary method for identifying wheat with different thousand grain weight properties, and special primer thereof
  • Auxiliary method for identifying wheat with different thousand grain weight properties, and special primer thereof
  • Auxiliary method for identifying wheat with different thousand grain weight properties, and special primer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. The protein and its coding genes related to the weight of wheat seed granules

[0031] 1. Tacwi-A1A protein and its coding genes obtained

[0032] The obtained method of TACWI-A1A protein encoding gene adopts the method of electronic cloning combined with conventional PCR amplification.According to the BLASTE retrieval of the GenbankAccessionNumber: EU095553) in the wheat EST database, the screened EST is spliced in the wheat EST database.PrimierPrimer5 software design 3 pairs of primers C1, C2, C3, and obtain a real CWI gene sequence from the genome DNA of wheat varieties.

[0033] The PCR reaction uses the wheat genome DNA as the template, which is performed on the MJResearchPTC-200pcr instrument. -1) 1.0 μl each, dntp (25 μmol·L -1 ) 0.2 μL, 2 μL of 10 × PCR buffer, and supplementing the reaction system with sterile distilled water to 20 μL.PCR reaction uses TouchDown to annealing procedures: first 95 ° C 5min; then 95 ° C 60s, starting from 66 ° C, the anneal...

Embodiment 2

[0042] Example 2. Analysis of the correlation between the weight of wheat seed granules and genotypes

[0043] 1. Design of functional marking primers

[0044] According to the sequences in the sequence list 2. The sequence 4 design two sets of primers CWI21 (primer pair B; P1 / P2) and CWI22 (primer to A; P3 / P2) are as follows:

[0045] P1 (upstream primer): 5’-gtggtgatggggtttttttaag-3 '(sequence 5);

[0046] P3 (upstream primer): 5’-GGTGATGAGAGTTCATGGTTAAT-3 '(Sequence 6)

[0047] P2 (downstream primer): 5’-AGAAGCCCAACAAAAAAACAAC-3 '(Sequence 7).

[0048] Use a primer to the B, which can expand the DNA fragment of 404bp in the sequence 4 in the sequence 4 in the serial list. The DNA fragment in the sequence 2 in the sequence 2 in the sequence list cannot be expanded.The DNA fragment of 402bp in the serial 2 in the list 2 cannot expand the DNA fragment in the sequence 4 in the sequence 4 in the sequence list.

[0049] 2. Analysis of the correlation between wheat seed grain weight a...

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Abstract

The invention discloses an auxiliary method for identifying wheat with different thousand grain weight properties, and a special primer thereof. The method comprises the following steps of: using a wheat gene group DNA as a template, and carrying out PCR enlargement by a primer pair A and a primer pair B, wherein if the enlargement product of the primer pair A has a 402bp DNA segment, while the enlargement product of the primer pair B does not have the 402bp DNA segment, the gene type of the wheat to be tested is TaCwi-Ala; if the enlargement product of the primer pair B has the 402bp DNA segment, while the enlargement product of the primer pair A does not have the 402bp DNA segment, the gene type of the wheat to be tested is TaCwi-Alb; and the thousand grain weight of the wheat with the gene type of TaCwi-Ala is greater than that of the wheat with the gene type of TaCwi-Alb in statistics. The invention can be used for culturing fine wheat varieties with high thousand grain weight, and avoid sieving the wheat varieties with low thousand grain weight. The wheat varieties can be sieved in the early period of growth, therefore, the hybridized combination is optimized, the breeding speed is accelerated, and the breeding cost is decreased.

Description

Technical field [0001] The present invention involves a method of assisting identification with different thousand -grain heavy wheat and its special primers. Background technique [0002] Wheat is one of the most important food crops in the world. It provides more than 20 % of human nutrients, and more than 35 % of the world's population is the main grain.my country's wheat planting area, total production and consumption ranks first in the world, and its output accounts for about 1 / 5 of my country's total grain output. [0003] Increasing wheat output is an important goal of wheat breeding.The unit area of the wheat is composed of the unit area of the unit area, the number of grains per ear, and the average granules.Thousands of weights have the greatest contribution to the output in the factors of yield composition. Increased average single -scooter weight under group conditions is the key to increasing the potential of variety group output.Studies have shown that each type of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/29C07K14/415
Inventor 夏先春马冬云何中虎
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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