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Paired human embryonic stem cell lines with same HLA property

A technology of human embryonic stem cells and embryonic stem cells, applied in the direction of microorganisms, biochemical equipment and methods, tissue culture, etc., can solve the problems of transmission of pathogens and foreign protein contamination, and achieve the effect of avoiding immune rejection

Inactive Publication Date: 2010-07-14
广州医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because MEFs are heterologous cells, there is a risk of spreading pathogens and causing foreign protein contamination when culturing hES with MEFs

Method used

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  • Paired human embryonic stem cell lines with same HLA property
  • Paired human embryonic stem cell lines with same HLA property
  • Paired human embryonic stem cell lines with same HLA property

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] 1. Establishment of human embryonic stem cell lines

[0037] Material

[0038] 1. Embryo source

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Abstract

The invention relates to a pair of human embryonic stem cell lines with the same HLA property. The paired human embryonic stem cell lines have the same HLA property, normal karyograms and higher affinities for expressing specific surface antigens; the paired human embryonic stem cell lines are strongly positive through AKP detection, and simultaneously, can express Oct-4 transcription factors and display undifferentiated properties; and the average population doubling time is about 32 hours. More choices for solving HLA mating difficulty are provided by researching the properties of the pair of human embryonic stem cell lines from the same male parent and the same female parent in biology, genetics and epigenetics, and meanwhile, the two embryonic stem cell strains provide good models for clinical basic research.

Description

technical field [0001] The present invention relates to pluripotent embryonic stem cells, in particular to a pair of human embryonic stem cell lines with the same HLA and derivatives thereof. Background technique [0002] Human embryonic stem cells (hES) are isolated and cultured from the inner cell mass (ICM) of blastocysts. They are cells with totipotent differentiation characteristics, self-renewal and multi-lineage differentiation potential. It is of great significance in the fields of therapy, embryonic development, drug screening, gene therapy, and research on genetic and epigenetic mechanisms. Human embryonic stem cell research began in 1998, and it has become the most active research field in life science after the Human Genome Project. In recent years, breakthroughs have been made in the isolation and purification, performance research, culture expansion, and induced differentiation of embryonic stem cells. In 1998, the Thomson laboratory of the University of Wisc...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 孙筱放
Owner 广州医学院
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