Culex and aedes attractant and application method
An attractant, Aedes mosquito technology, applied in the direction of attracting pests, botanical equipment and methods, application, etc., to achieve the effect of improving the attracting effect, reducing the number, and reducing the probability of harassment and stings
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Embodiment 1
[0022] In the laboratory, octanoic acid 10 μg / mL and indole 0.1 μL / mL were mixed at a volume ratio of 1:1 as an attractant. The "T" type olfactometer (refer to the design of Geier et al., 1996 with some modifications) was used to measure the attractant's attractant effect on female adults of Culex pachypis. The base arm of the "T" type olfactometer is 35cm long, and the two "T" arms are each 20cm long and 7cm in diameter. Connect the base arm to the mosquito release chamber; two sample chambers are extended from the ends of the two "T" arms, and the airflow is connected to the sample chamber through the "Y" pipe. Before the air flow enters the sample chamber, the air is first filtered by activated carbon and humidified with distilled water, and the gas flow in the olfactometer is adjusted to 900ml / min. The room temperature is 26-28°C, the relative humidity is 70-80%, and the light intensity in the olfactometer is 100-150 lux. Use a mosquito suction tube to suck 30 female mos...
Embodiment 2
[0026] In the laboratory, octanoic acid 10 μg / mL and indole 0.1 μL / mL were mixed at a volume ratio of 1:1 as an attractant. The "T" type olfactometer was used to measure the attracting effect of compounds on female adults of Aedes albopictus. The test method is the same as above.
[0027] Results: The arm end of the attractant can attract about 88% of mosquitoes. The female Aedes albopictus mosquitoes reacted towards the attractant, and the attractant had obvious attracting effect on the female Aedes albopictus mosquitoes.
Embodiment 3
[0029] Field release test. This experiment was carried out around the human house in Xiaokeyi Village, Xundian County, Kunming City in August 2009. The mosquito trap MT-1 (GB / T5836.2) jointly manufactured by the Institute of Microbial Epidemiology of the Chinese Academy of Military Medical Sciences and Beijing Longguan Technology Development Co., Ltd. is used. CO 2 The air flow through the fan blows the mosquitoes into the bag. Caprylic acid and indole were mixed with ethanol to a concentration of 10 μg / mL and 1 μL / mL, mixed at a volume ratio of 1:1, put into a glass bottle, and hung beside a mosquito trap. Trap from 5:00pm to 10:00pm. Attractant+CO 2 Mosquito traps and CO 2 The mosquito trap lamps were compared at the same time, and the test was carried out for 3 days, and the mosquito trap results of the 3 days were averaged. The results are as follows:
[0030]
[0031] In the above table, the number of mosquitoes captured by the attractant is used as the trapping ...
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