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Preparation method of agarose gel microfluidic device

An agarose gel, microfluidic device technology, applied in chemical instruments and methods, laboratory containers, chemical/physics/physicochemical processes, etc. Chip and other problems, to achieve the effect of good sealing and simple operation

Inactive Publication Date: 2012-07-11
CHONGQING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the way of punching holes in the vertical direction of the channel is not suitable for microfluidic chips with agarose gel as the main material. Due to the poor shrinkage of agarose gel, punching holes will lead to poor sealing and serious leakage. Phenomenon

Method used

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  • Preparation method of agarose gel microfluidic device
  • Preparation method of agarose gel microfluidic device
  • Preparation method of agarose gel microfluidic device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1 Preparation of agarose gel microfluidic device

[0028] a arrange microfilaments:

[0029] Two inlets (2) and two outlets (3) with a diameter of 2 mm are respectively cut on the side walls of the front and rear sides of a mouth-shaped plexiglass frame (1) with an inner diameter of 4 to 6 cm and a width of 2 to 3 cm. , insert the liquid inlet pipe (4) and liquid outlet pipe (5) with a diameter of 2 mm into the mouth-shaped plexiglass frame (1) through the above-mentioned 2 inlets (2) and 2 outlets (3), see figure 1 ; The liquid inlet pipe (4) and the liquid outlet pipe (5) are connected by a filament (6) with a diameter of 1 mm, see figure 2 ;

[0030] b pouring solidification:

[0031] Pad slide glass (7) below the square-shaped plexiglass frame (1), and use clamps to fix the square-shaped organic glass frame (1) and slide glass (7), and put the agar with a mass concentration of 3% The sugar solution is heated and melted, and slowly introduced into the square-sha...

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Abstract

The invention relates to the field of making biochips, in particular to a preparation method of an agarose gel microfluidic device. Particularly, the agarose gel microfluidic device is prepared by three steps of assigning microfilaments, pouring and solidifying, and wiredrawing. The agarose gel microfluidic device manufactured by the method has good sealing property, and the recovery rate of liquid is up to 95%. The agarose gel microfluidic device manufactured by the method can generate stable concentration gradients, and is used for observing interaction between cells and biochemical micro-environments, especially the interaction under a flow condition. The method has simple operation, and the microfluidic device can be manufactured in an ordinary lab without expensive precision instruments.

Description

technical field [0001] The invention relates to the field of biochip production, in particular to a preparation method of an agarose gel microfluidic device. Background technique [0002] Agarose gel is a substance with a porous network structure, which allows macromolecular substances (molecular weight from hundreds of thousands to several million) to pass through freely, so it can be used as a good diffusion medium in various biological experiments; Agarose gel can not only ensure the diffusion balance of the concentration gradient in a short period of time, but also has the coordination of rigidity and toughness; in addition, the agarose gel has high transparency and has the advantage of good light transmission and easy observation compared with other gels. Microfluidic technology is a research hotspot in life sciences in recent years, but few people choose agarose gel as the matrix material of microfluidic devices. [0003] Shing-Yi Cheng. A hydrogel-based microfluidic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01J19/00
Inventor 熊新黎昌莉蔡绍皙邹米莎
Owner CHONGQING MEDICAL UNIVERSITY
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