Poultry IL-2 and newcastle disease virus HN gene recombination fusion protein and application thereof

Abstract

The invention relates to a preparation method and application of fusion protein (chIL-2-HN fusion protein) of recombining poultry interleukin-2 (IL-2) and newcastle disease virus hemagglutinin-neuraminidase (HN), belonging to the technical field of biological engineering. The fusion protein is fused by poultry IL-2 protein and newcastle disease virus HN protein by flexible Linker peptide; a DNA sequence for coding the fusion protein is inserted into an expression vector pPICZ alpha A; saccharomycete X-33 is electrically converted to obtain a genetically engineered microorganism for efficiently expressing recombination chIL-2-HN fusion protein which is prepared by liquid culture and purification; the recombination fusion protein can serve as the novel genetic engineering vaccine and can serve as a novel immunologic adjuvant for newcastle disease common vaccine. The chIL-2-HN fusion protein of the invention has favourable safety and no toxic or side effect, which is verified by experiments of animal immunoassay. In addition, the chIL-2-HN fusion protein can effectively strengthen the level of organism cellular immunity and humoral immunity and has wide application prospect.

Description

technical field The present invention relates to the fusion protein of poultry interleukin-2 (Interleukin-2, IL-2) and Newcastle disease virus HN, the DNA sequence encoding the fusion protein, the vector and the host cell containing the DNA sequence, and the recombinant fusion The protein and its application belong to the technical field of genetic engineering production of vaccines and immune adjuvants in the biotechnology pharmaceutical industry. Background technique Newcastle disease (ND) is caused by Newcastle disease virus (NDV) and is a highly contact, acute disease of poultry characterized by dyspnea, yellow-green loose stools, neurological disorders, and mucosal and serosal hemorrhage. Septic infectious disease. Listed by the International Office of Epizootics as one of the most harmful Class A infectious diseases to animals, it has caused huge economic losses to the poultry industry. Newcastle disease is the number one disease that threatens the poultry industry d...

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Problems solved by technology

Therefore, with the rapid development of molecular biology technology in recent years, the development of NDV genetically engineered vaccines has become a hot spot for people to stu

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