A human non-antibody peptide or protein phage library

A phage and protein technology, applied in the field of human non-antibody peptide or protein phage library, can solve the problem that peptides have not yet been reported.

Inactive Publication Date: 2011-02-09
CENTOCOR ORTHO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous reports on pIX phage display described fusions in the context of phagemid vectors; display of polypeptides on pIX from hybrid vectors or phage vectors has not been reported

Method used

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  • A human non-antibody peptide or protein phage library
  • A human non-antibody peptide or protein phage library
  • A human non-antibody peptide or protein phage library

Examples

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example 1

[0055] Example 1: Construction of an exemplary engineered phage vector

[0056] Construction of type 9 phage vector: The prototype M13-9 vector (PHEPP208) was constructed to contain the signal sequence (pelB) from pectate lyase B and the double BbsI restriction enzyme recognition site for the M13KE in the phage genome Subsequent clones were inserted between the pVII and pIX genes in (derivative of M13mp19). In the unmodified M13KE phage genome, the terminal nucleotide base of the last amino acid codon of the pVII gene is the first nucleotide base of the ATG initiation codon of the pIX gene. This last and first nucleotide shared between the pVII and pIX genes was retained in PHPEP 208 between the pVII gene and the ATG start codon of the peIB signal sequence. The influenza hemagglutinin (HA) peptide YPYDVPDYA and the nine-amino acid linker SGGSGGTKT are included between the eIB signal sequence and gene pIX. Three other peptides (Table 1) and the small globular protein epider...

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Abstract

The invention relates to a compositions and methods for generating and using pIX phage display libraries for producing non-antibody peptide or protein proteins or peptides using engineered hybrid phage vectors derived from pIX of M 13 phage.

Description

technical field [0001] The present invention relates to compositions and methods for generating a pIX phage display library using engineered hybrid phage vectors derived from pIX of M13 bacteriophage and using the library to generate one or more non-antibody peptides or proteins. Background technique [0002] Phage display is a well-established tool for affinity-based peptide selection. In a typical phage display selection, a library of peptides is engineered to be fused to the terminus of one of the coat proteins of the filamentous bacteriophage M13. Phage particles provide the physical association between each polypeptide member of the library and the gene it encodes. The phage library can then be subjected to affinity selection, or panning, for library members that bind to the desired target molecule. The library is mixed with target molecules, unbound phage particles are washed away, and the remaining phage are eluted and amplified by culturing in E. coli cells. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/09
CPCC40B40/02C12N15/1037
Inventor L·尹C·黄K·奥奈尔
Owner CENTOCOR ORTHO BIOTECH
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