Antrodia camphorata cyclohexenone compound for inhibiting growth of pancreatic cancer tumor cells

A tumor cell and compound technology, applied in the field of antrodia cyclohexenone compound used to inhibit the growth of pancreatic cancer tumor cells, can solve unpublished specific problems and the like

Active Publication Date: 2011-04-06
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its constituents have been analyzed one after another, no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomum. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cintrodia Cintrodia anticancer, and to apply Antrodia Cinnamomea to cancers such as pancreas. Dirty cancer treatment and prevention are of great help

Method used

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  • Antrodia camphorata cyclohexenone compound for inhibiting growth of pancreatic cancer tumor cells
  • Antrodia camphorata cyclohexenone compound for inhibiting growth of pancreatic cancer tumor cells
  • Antrodia camphorata cyclohexenone compound for inhibiting growth of pancreatic cancer tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.

[0025] The Antrodia camphorata extract collected above was analyzed using a high performance liquid chromatography (High Performance Liquid chromatography) with a RP18 column (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) were used as Mobile phase (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is 10% ~ 90%; 35 ~ 40 minutes, ...

Embodiment 2

[0028] Anti-pancreatic cancer tumor cell activity test in vitro

[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium of human pancreatic cancer tumor cells BxPC-3 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the conventional MTT assay method, and the pancreatic cancer tumor cell BxPC-3 is a human pancreatic adenocarcinoma cell line (ductal adenocarcinoma cell line).

[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is ...

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Abstract

The invention relates to a new purpose of a compound. In the invention, antrodia camphorata extract is separated and purified to obtain 4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone. The cyclohexenone compound can be used for inhibiting growth of pancreatic cancer tumor cells and simultaneously can be used for pharmaceutical compositions for inhibiting growth of pancreatic cancer tumor cells.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from antrodia cinnamomea (Antrodia cinnamomea) extract to inhibit the growth of pancreatic cancer tumor cells. Background technique [0002] Pancreatic cancer is the fourth leading cause of cancer death in Western countries, and it is also the tenth among the top ten causes of cancer death in Taiwan. About 60% of pancreatic cancers occur in the head of the pancreas, and about 21% may invade the entire pancreas. Among exocrine pancreatic tumors, pancreatic ductal adenocarcinoma (pancreatic ductal adenocarcinoma) is The most common type, accounting for 85-90% of all pancreatic cancers. [0003] Pancreatic cancer is a malignant disease with strong aggressiveness and high fatality. The treatment of this kind of cancer mainly includes surgical treatment, chemotherapy and radiotherapy. % of patients have the opportunity to undergo su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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