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Method for producing polysaccharide by crossly culturing tremella fuciformis basidiospores by stages

A tremella spore, stage-based technology, applied to the field of cross-stage culture of tremella spores to produce polysaccharides, to achieve the effect of increasing yield and simple cultivation process

Inactive Publication Date: 2013-07-24
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of using three-stage cross-cultivation to increase the yield of tremella polysaccharide has not been reported in the literature, so it has a good development prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: culture temperature, rotating speed are the same as comparative example. Rotary culture is used in the first stage of fermentation (0-1.5d), rotary culture is used in the second stage of fermentation (1.5-3.0d), and reciprocating culture is used in the third stage of fermentation (3.0-4.5d). Tremella polysaccharides were fermented and produced by cross-cultivation strategy, and the yield increased to 2.14g / L.

Embodiment 2

[0017] Embodiment 2: culture temperature, rotating speed are the same as comparative example. Rotary culture is adopted in the first stage of fermentation (0-1.5d), reciprocating culture is adopted in the second stage of fermentation (1.5-3.0d), and reciprocating culture is adopted in the third stage of fermentation (3.0-4.5d). Tremella polysaccharides were fermented and produced by cross-cultivation strategy, and the yield increased to 3.93g / L.

Embodiment 3

[0018] Embodiment 3: culture temperature, rotating speed are the same as comparative example. In the first stage of fermentation (0-1.5d), reciprocating culture was adopted, in the second stage of fermentation (1.5-3.0d) rotary culture was adopted, and in the third stage of fermentation (3.0-4.5d) rotary culture was adopted, using these three stages Tremella polysaccharides were fermented and produced by cross-cultivation strategy, and the yield increased to 3.64g / L.

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PUM

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Abstract

The invention discloses a method for producing polysaccharide by crossly culturing tremella fuciformis basidiospores by stages, comprising the following step that the fermenting and culturing process includes three stages which are respectively carried out on a rotary type swing bed and a reciprocated type swing bed at the same rotation speed for crossly culturing. By applying the method providedby the invention for culturing tremella fuciformis conidiums, tremella fuciformis polysaccharide can be produced in high efficiency, and the output of the polysaccharide can reach 5.80g / L. The method provided by the invention is a production method with favorable industrialized prospect, thereby laying the foundation for producing tremella fuciformis polysaccharide for the industrialized economy.

Description

technical field [0001] The invention belongs to the field of biological engineering and technology. The invention relates to a production process of tremella polysaccharides, in particular to a process for producing polysaccharides by cross-stage culturing tremella spores. Background technique [0002] Tremella fuciformis (Tremella fuciformis) is a higher fungus of the class Laminaria, Tremellaceae, and Tremella genus. The main medicinal ingredients of white fungus are polysaccharides, mainly including acid polysaccharides, neutral heteropolysaccharides, acid oligosaccharides, cell wall polysaccharides, etc. Tremella polysaccharide has the functions of strengthening the heart, delaying aging, anti-thrombosis, lowering blood fat, lowering blood sugar, anti-hepatitis, anti-mutation, anti-tumor, improving immunity, and anti-radiation. Tremella polysaccharide has a wide range of applications in medicine, beauty, health food and other fields. Tremella polysaccharides can be is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 朱虎张帅帅
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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