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Device and method for preparing stem cells through continuous perfusion bioreactor/tank (bag) system

A bioreactor and cell technology, applied in bioreactor/fermenter combinations, specific-purpose bioreactor/fermenter, biochemical equipment and methods, etc., can solve problems such as limited single factors, to save manpower, Low impact, high intensification effect

Active Publication Date: 2011-05-25
浙江新生泉细胞科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, similar studies only focus on the problem of limited single factors in the process of stem cell expansion, and have not been able to use continuous perfusion bioreactors combined with effective monitoring methods to perform efficient, continuous, controllable, and engineerable amplification of stem cells. amplified preparation

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  • Device and method for preparing stem cells through continuous perfusion bioreactor/tank (bag) system
  • Device and method for preparing stem cells through continuous perfusion bioreactor/tank (bag) system

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Embodiment Construction

[0027] As shown in the figure, the device and method for preparing stem cells by continuous perfusion bioreactor and culture bottle / bag system, including culture solution storage tank / bag 1, monitorable bioreactor 2, central cell culture chamber 3, waste liquid / harvesting liquid Collection container 4, bioreactor physical and biochemical index detection head group 5, cell culture warehouse entry physical and biochemical index detection head group 6, cell culture warehouse exit physical and biochemical index detection head group 7, online monitoring system 8, No. 1 fluid control Speed ​​pusher 9, No. 2 fluid speed control pusher 10, controllable speed pipeline system 11. The connection relationship is as follows: the culture solution storage tank / bag 1 is connected to the monitorable bioreactor 2 through the speed control pipeline system 11 through the No. 1 fluid speed control pusher 9; the monitorable bioreactor 2 passes through the speed control pipeline body 11 , connected ...

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Abstract

The invention relates to a device for preparing stem cells through a continuous perfusion bioreactor / culture bottle (bag) system. The device comprises a culture solution storage tank / bag, a monitored bioreactor, a central cell culture warehouse, a waste liquor / obtained liquor collecting container, physicochemical and biochemical indicator detecting head groups, an online monitoring system and a sterile filter membrane filter, which are connected by a speed-controlled pipeline system of a fluid-controlled sterile driver. The connecting relationships are that: the culture solution storage tank / bag is connected with the monitored bioreactor through the speed-controlled pipeline system; the monitored bioreactor is connected with an inlet of the central cell culture warehouse through the speed-controlled pipeline system; an outlet of the central cell culture warehouse is connected with the waste liquor / obtained liquor collecting container through the speed-controlled pipeline system; the monitored bioreactor is connected to the online monitoring system through a physicochemical and biochemical indicator detecting head group of the reactor; and the online monitoring system is connected with the physicochemical and biochemical indicator detecting head groups at the inlet and outlet of the central cell culture warehouse respectively.

Description

technical field [0001] The present invention relates to a device and method for preparing stem cells in a continuous perfusion bioreactor and a culture bottle / bag system in the field of bioengineering, in particular to a continuous perfusion bioreactor and a culture bottle / bag system that are valuable but difficult to grow Devices and methods for stem cells. Background technique [0002] Judging from the current progress of tissue engineering, the use of stem cells to treat tissue and organ damage and necrosis is the most promising treatment method, and its medical effect and economic return expectations are considerable. So far, stem cells (such as mesenchymal stem cells) are representative of the therapeutic cell culture preparation method. Traditionally, the stem cells are extracted and inoculated on a plate or T bottle, and placed in a 37°C, saturated humidity, 5% carbon dioxide incubator. Perform static discontinuous feed culture. However, the preparation of stem cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/24C12N5/074C12N5/0775
CPCC12M29/04C12M41/00C12M23/14
Inventor 齐瀚实郑琛李巍巍
Owner 浙江新生泉细胞科技有限公司
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