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Preparation method of myocardial ischemia/reperfusion model in rats

A technique of myocardial ischemia and reperfusion, which is applied in the fields of basic medical science and clinical research modeling, can solve problems such as the inability to maintain a constant ischemia effect, the inability to simulate the actual clinical situation well, and the deviation of measured data from the real situation. Achieve clinical ischemia/reperfusion injury, prevent external force pulling, and avoid secondary thoracotomy

Inactive Publication Date: 2011-06-01
TAIYUAN IRON & STEEL GROUP
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AI Technical Summary

Problems solved by technology

This method has the following disadvantages. First, the position of the tube changes due to the beating of the heart, which cannot guarantee a constant ischemic effect during the experiment; second, the cut surface of the tube touches the surface of the heart, and the myocardium suffers serious mechanical damage; The compression of the coronary artery by the clamp maintenance tube not only increases the damage, but also the heart is pulled by external force, which cannot simulate the actual clinical situation well, especially when it is necessary to dynamically monitor the heart function of animals, the measured data may deviate from the real situation
The compression tube method is to place the tube on the surface of the heart, tie the wire to the tube to block the coronary artery to form ischemia, and withdraw the tube to restore blood perfusion when the ischemia ends. However, due to the difficulty of fixing the tube and poor ischemia effect, it is rarely used

Method used

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  • Preparation method of myocardial ischemia/reperfusion model in rats
  • Preparation method of myocardial ischemia/reperfusion model in rats
  • Preparation method of myocardial ischemia/reperfusion model in rats

Examples

Experimental program
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Embodiment 1

[0025] In this embodiment, a total of 60 male Wistar rats were divided into two groups, an ischemia / reperfusion group and a sham operation group, with 30 rats in each group.

[0026] ischemia / reperfusion group

[0027] In this example, 30 male Wistar rats born at 8-10 weeks and weighing 180-220 g were subjected to ischemia for 30 minutes and reperfusion for 3 hours as an example to illustrate the preparation method of the rat myocardial ischemia / reperfusion model. Each rat is anesthetized according to the following sequential steps, the chest skin is cut open to extrude the heart, the left anterior descending coronary artery is ligated and the ligation thread is loosened, and the steps are characterized by:

[0028] 1. Anesthesia

[0029] Put the rats in the induction box of the Matrx small animal anesthesia machine, and after induction of anesthesia with 2% to 3% isoflurane, take the rats out of the induction box, and continue to administer isoflurane with an anesthesia mask...

Embodiment 2

[0062] In this embodiment, a total of 60 male Wistar rats were divided into two groups, 30 in each group.

[0063] ischemia / reperfusion group

[0064] In this example, 30 male Wistar rats born at 8-10 weeks and weighing 180-220 g were ischemiaed for 30 minutes and reperfused for 24 hours as an example to illustrate the preparation method of the rat myocardial ischemia / reperfusion model. Each rat is anesthetized according to the following sequential steps, the chest skin is cut open to extrude the heart, the left anterior descending coronary artery is ligated and the ligation thread is loosened, and the steps are characterized by:

[0065] 1. Anesthesia

[0066] Put the rats in the induction box of the Matrx small animal anesthesia machine, and after induction of anesthesia with 2% to 3% isoflurane, take the rats out of the induction box, and continue to administer isoflurane with an anesthesia mask during the subsequent operation Alkane inhalation was used to maintain anesth...

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Abstract

The invention relates to a preparation method of myocardial ischemia / reperfusion model in rats. The preparing method comprises the following steps in turn: I, anesthetizing, namely placing rats in an induction box, performing inhalation anesthesia by using 2 to 3 percent isoflurane, taking the rats out of the induction box, allowing the rats to continuously inhale isoflurane under a mask to maintain anesthesia; II, cutting off the chest skin and extruding the heart, disinfecting the chest skin, cutting off the chest skin, expanding the subcutaneous muscle to enter the chest, and directly extruding the heart out of the intercostals; III, ligating the left anterior descending coronary artery, ligating a starting end of the left anterior descending coronary artery by using a slipknot, sending the heart back to the chest after ligation, making one end of the ligating line positioned out of the chest, sewing and closing the chest; and IV, loosening the ligating line after ischemia is finished, directly loosening the ligating line exposed out of the chest, and restoring blood supply in the coronary. The invention is simple in operation, short in time consumption and relatively high in survival rate of the rats.

Description

technical field [0001] The invention relates to the modeling of medical foundation and clinical research, in particular to the preparation method of rat myocardial ischemia / reperfusion model. Background technique [0002] As we all know, the most effective treatment for acute myocardial infarction is coronary artery reperfusion. However, there is reperfusion injury in coronary artery reperfusion. Therefore, the research and prevention of reperfusion injury has become a hot research topic. Blocking the left anterior descending coronary artery in animals to cause acute myocardial ischemia, and restoring blood supply for reperfusion within a predetermined time is a necessary experimental method for simulating human ischemic heart disease to restore blood supply. Rats are the preferred experimental animals for making myocardial ischemia / reperfusion injury models due to their advantages such as less coronary collateral circulation, early myocardial necrosis, good repeatability an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61D1/00A61D7/04
Inventor 梁峰燕子温永金曹成章
Owner TAIYUAN IRON & STEEL GROUP
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