Latex microsphere with amide group on surface and preparation method of latex microsphere

Abstract

The invention discloses a latex microsphere with an amide group. The latex microsphere is prepared from the following raw materials by weight percent: 1.2%-11.1% of aliphatic conjugated diene, 16.7%-65.3% of nitroaromatic compound, 19%-65% of alkenyl unstaturated amide, 0.04%-10.8% of a surface active agent, 0-19.4% of an initiator and 4.3%-29.1% of a stabilizing agent. The invention also discloses a preparation method of the latex microsphere with the amide group. An incident light with a wavelength of 200-800 nm is used to irradiate the latex microsphere disclosed by the invention, so that good scattered light is obtained at the position which forms an included angle of 15 degrees-60 degrees with the incident light. When the latex microsphere labeled by an antibody is mixed with an antigen, a large immune compound is formed due to the cross-linked reaction between the antigen and the antibody, so as to cause the variation of scattered light strength in different directions. The detection of a target object can be obtained through the variation of the scattered light strength, thus better specificity and accuracy are embodied, and the latex microsphere is safe and has no toxicity.

Description

technical field [0001] The invention relates to a detection reagent for labeling an antigen or an antibody in an immune experiment and a preparation method thereof. Background technique [0002] Latex microspheres are a detection reagent that can be used to label antigens or antibodies in immunoassays to detect the reactants in target samples. Most latex detection reagents on the market now use latex microspheres without functional groups and latex microspheres with carboxyl functional groups. The surface of latex microspheres without functional groups contains abundant hydrophobic sites, which will non-specifically adsorb various proteins, and as the system environment changes, dissociation and modification will occur, resulting in unstable properties of the reagent. Latex microspheres with carboxyl functional groups are connected to the amino site of the protein when they bind to the antibody. This site belongs to the specific affinity site of the antibody. decline. In ...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of inaccurate and unsafe detection of existing in vitro immunoassay reagents, the present invention provides a latex microsphere with amide groups on the surface and a preparation method thereof