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Spectrum biosensing device

A bio-sensing and spectroscopic technology, applied in measurement devices, material analysis by optical means, instruments, etc., can solve the problems of prolonged detection time, ineffective use of spatial information, and increased complexity of detection systems. Effects of complexity, good accuracy and specificity

Pending Publication Date: 2020-12-11
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the entire microfluidic system undoubtedly greatly increases the detection cost and operational difficulty.
In addition, it is also a solution to use the spatial movement of the spectrometer to complete the collection of spectra on a series of sites, but the mechanical movement has caused the prolongation of the detection time, and the accompanying mechanical control device has also increased the complexity of the entire detection system (CN208705218U Multi-channel absorption spectrum detection platform and detection system)
Therefore, in the field of biosensing, the spatial information of spectral imaging has not been effectively utilized.

Method used

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  • Spectrum biosensing device
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Examples

Experimental program
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Embodiment 1

[0049] This embodiment provides a spectral biosensing device, including: LSPR nanobiological chip, spectral imaging module and data processing module;

[0050] Wherein, the LSPR nano biochip (vertical structure such as image 3 (shown in Figure A) includes: a base material 1, a nanomaterial 2 with LSPR effect disposed on the base material, and biomolecules 3 modified on the surface of the nanomaterial.

[0051] Specifically, the base material is glass.

[0052] The nanometer material is a metal particle with a periodic nanometer structure, and is distributed on the base material in the form of an array, and different biomolecules are decorated on the nanometer material at different array positions.

[0053] The biomolecules are bound to the surface of the nanomaterials via chemical covalent bonds (mercapto-gold). First, PEG carboxylic acid molecules with sulfhydryl groups form a unified self-assembled molecular layer at the key hydrophilic sites of gold nanofilms through thi...

Embodiment 2

[0058] This embodiment provides a spectral biosensing device, including: LSPR nanobiological chip, spectral imaging module and data processing module;

[0059] Wherein, the LSPR nano biochip (vertical structure such as image 3 (shown in Figure B) includes: a base material 1, a nanomaterial 2 with LSPR effect disposed on the base material, and biomolecules 3 modified on the surface of the nanomaterial.

[0060] Wherein, the nanomaterial 2 is a gold nanofilm (nanopore) with periodic nanostructure holes ( Image 6 ), which are divided into critical regions 21 and non-critical regions 22, only the nanomaterials in the critical region 21 are modified with biomolecules, and the biomolecules modified on the nanomaterials in different critical regions 21 are different. The other part is the non-critical area 22, which can be made of other materials, and can also communicate with the circulation layer.

[0061] Specifically, the base material is glass.

[0062] The biomolecules are...

Embodiment 3

[0067] This example provides a detection method using the spectral biosensing device in Example 2, aiming at the detection of different viruses or their representative molecules.

[0068] When using the described spectral biosensing device to carry out the detection of different viruses or their representative molecules, the following processes are included (with reference to Figure 4 ):

[0069] (1) Preparation of standard curves for different substances to be tested

[0070] Before the test, the spectral image of the LSPR nano-biological chip in the flow cell will be firstly recorded by the spectral imaging device as a reference spectral image. By sequentially injecting different concentrations (ranging from 0.01pg / mL to 10mg / mL) of standard substances to be tested (including S protein, N protein, and specific nucleic acid molecules of the new coronavirus, SARS, and MERS viruses; HA proteins of different influenza viruses, Specific nucleic acid molecules, etc.), and sampl...

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Abstract

The invention relates to the field of molecular detection, in particular to a spectrum biosensing device. Based on the optical property of an LSPR nano material and in combination with effective spatial information in spectral imaging, an LSPR nano biochip generally with single-channel single-substance detection capability has the capability of simultaneously detecting various different substances. Meanwhile, rapid and accurate detection and high-throughput screening can be carried out only by the nano biochip and a spectral imaging device, so that the complexity, the use cost and the operation difficulty of a detection system with similar functions are greatly reduced.

Description

technical field [0001] The invention relates to the field of molecular detection, in particular to a spectral biosensing device. Background technique [0002] Since the 1980s, surface plasmon resonance technology has been widely used to track the interaction between biomolecules in real time, and its characteristic is that it does not require any labeling. Later, with the development of micro-nano processing technology and imaging equipment, localized surface plasmon resonance (LSPR: Localized Surface Plasmon Resonance) technology also emerged as the times require, providing support for portable related equipment and multi-functional chip design. The LSPR phenomenon is the effect that light energy is absorbed when light of a specific wavelength is incident on a metal material with a nanostructure and resonates with the overall vibration frequency of its surface electrons. Generally, nanomaterials composed of noble metals such as gold, silver, platinum, etc. have a strong LS...

Claims

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Application Information

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IPC IPC(8): G01N21/552G01N21/59
CPCG01N21/554G01N21/59G01N2021/5903
Inventor 刘啸虎王毅张庆文
Owner WENZHOU MEDICAL UNIV
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