Cultivation method of lucilia sericata larva and disinfection method thereof

A technology of Lucilia sericata and a disinfection method, which is applied in the field of cultivation and disinfection of live insects, can solve the problems of artificial breeding, breeding and disinfection without mature methods, unfavorable passage, and death of eggs, so as to maintain the vitality of insects and facilitate Easy to master and operate

Inactive Publication Date: 2012-10-03
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lucilia sericata goes through four stages in its life: eggs, larvae (maggots), pupae, and adults (flies). Its larval stage can be used as a medical insect, has scavenging properties, and has a strong ability to reproduce and grow in the natural environment, but it is artificially raised indoors. , There is no mature method for reproduction and disinfection
At present, there is a combination of sulfurous acid and formaldehyde to disinfect the eggs, and then hatch them in a sterile environment, raise them with sterilized food, and apply them clinically, so as to avoid secondary infection, but use sulfurous acid and formaldehyde to disinfect the eggs It will lead to an increase in the probability of egg death, which is not conducive to stable passage
There is also application of 3% creol soap solution to sterilize the eggs, and aseptic rearing and subculture, but there is still a 20-30% mortality rate

Method used

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  • Cultivation method of lucilia sericata larva and disinfection method thereof
  • Cultivation method of lucilia sericata larva and disinfection method thereof
  • Cultivation method of lucilia sericata larva and disinfection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Lucilia sericata egg collection:

[0041] Put the commercially available blue-spotted mackerel (Spanish mackerel) in the place where Lucilia sericata is infested, and collect the egg mass produced in its fish order;

[0042] 2. Disinfection of Lucilia sericata eggs:

[0043] ①Immerse the egg masses in 0.5% sodium sulfite solution and shake for 3 minutes to separate the adhered egg masses from each other, and take out the fish eyes with tweezers;

[0044] ② Filter out the liquid through a sterile filter, immerse the eggs in 5% sodium hypochlorite solution for disinfection for 3 minutes, and observe under a 13X microscope that all the eggs are alive;

[0045] ③Eggs were rinsed with sterile saline for 3 times;

[0046] ④ Sterile absorbent paper absorbs the moisture on the surface of the eggs;

[0047]⑤Take a 500ml aseptic porcelain jar as an insect breeding jar, put 50g of fish eye meal that has been ground from high-temperature sterilized carp fish eyes, and 40ml of...

Embodiment 2

[0060] 1. Lucilia sericata egg collection:

[0061] Put the commercially available blue-spotted mackerel (Spanish mackerel) in the place where Lucilia sericata is infested, and collect the egg mass produced in its fish order;

[0062] 2. Disinfection of Lucilia sericata eggs:

[0063] ①Immerse the egg masses in 1% sodium sulfite solution and shake for 5 minutes to separate the adhered egg masses from each other, and take out the fish eyes with tweezers;

[0064] ② Filter out the liquid through a sterile filter, immerse the eggs in 6% sodium hypochlorite solution for disinfection for 5 minutes, and observe under a 13X microscope that all the eggs are alive;

[0065] ③Eggs were rinsed with sterile saline for 5 times;

[0066] ④ Sterile absorbent paper absorbs the moisture on the surface of the eggs;

[0067] ⑤Take a 500ml aseptic porcelain jar as an insect breeding jar, put 50g of fish eye meal that has been ground from high-temperature sterilized carp fish eyes, and 40ml of ...

Embodiment 3

[0080] 1. Lucilia sericata egg collection:

[0081] Put the commercially available blue-spotted mackerel (Spanish mackerel) in the place where Lucilia sericata is infested, and collect the egg mass produced in its fish order;

[0082] 2. Disinfection of Lucilia sericata eggs:

[0083] ①Immerse the egg masses in 0.8% sodium sulfite solution and shake for 4 minutes to separate the adhered egg masses from each other, and take out the fish eyes with tweezers;

[0084] ②Filter out the liquid with a sterile filter, immerse the eggs in 5.5% sodium hypochlorite solution for disinfection for 4 minutes, and observe under a 13X microscope that all the eggs are alive;

[0085] ③Eggs were rinsed 4 times with sterile saline;

[0086] ④ Sterile absorbent paper absorbs the moisture on the surface of the eggs;

[0087] ⑤Take a 500ml aseptic porcelain jar as an insect breeding jar, put 50g of fish eye meal that has been ground from high-temperature sterilized carp fish eyes, and 40ml of ster...

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PUM

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Abstract

The invention provides a cultivation method of lucilia sericata larva and a disinfection method thereof, in particular to the disinfection to the egg and the larva in the process of cultivating. The disinfection method of the egg comprises the steps of: dipping the egg masses into 0.5-1% sodium sulfite solution, shaking at 3-5 minutes, so that the cohesive egg masses are separated from one another, and dipping the egg into 5-6% sodium hypochlorite solution to disinfect at 3-5 minutes after filtering; and the disinfection method of the larva comprises the step of disinfecting the larva by 0.3-0.5% iodophor solution and 0.1% bromo-geramine solution at two steps. The methods less influence the vitality of the fly egg, the death rate is 0%, the disinfection action to the larva is fast and durable, the toxicity is low, and the methods are less influenced by the organic matter; and the effective passage of the larva can be kept, the vitality of the disinfected lucilia sericata larva is kept, and the methods are simple to operate, easy to grasp, and suitable for popularization and application on a large scale.

Description

technical field [0001] The invention relates to a method for cultivating insects and disinfecting live insects, in particular to a method for cultivating and disinfecting Lucilia sericata larvae. Background technique [0002] The insect larvae used clinically to treat infected wounds are mainly the larvae of Lucilia sericata. Lucilia sericata, also known as the blowfly, is distributed worldwide. Belongs to Diptera, adult fly body length 5mm ~ 10mm. The body color is green with metallic luster, the cheeks are silvery white, the mane on the middle thorax is developed, and the axillary flap is hairless. There are more than 6 small hairs in the back of the shoulder mane, at the narrowest part of the forehead. The length of the stem of the fifth web is greater than 1 / 2 of the length of the lateral leaves. Anal lobe posteriorly tapering apex to terminal. Lucilia sericata goes through four stages in its life: eggs, larvae (maggots), pupae, and adults (flies). Its larval stage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 吕德成王寿宇张振
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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