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Preparation method of cassia bark extract, cassia bark extract and composition and application thereof

A technology of cinnamon extract and composition, which is applied in the field of preparation of drugs or health care products with dual effects and liver function, and the preparation of cinnamon extract, which can solve problems such as unclear mechanism of action, and achieve improvement of liver function, Effects of lowering blood sugar and increasing sensitivity

Active Publication Date: 2011-07-06
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, scientists in the United States and Germany have discovered that cinnamon has the effect of treating diabetes, and have achieved success in both mice and humans, but the mechanism of action is still unclear so far

Method used

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  • Preparation method of cassia bark extract, cassia bark extract and composition and application thereof
  • Preparation method of cassia bark extract, cassia bark extract and composition and application thereof
  • Preparation method of cassia bark extract, cassia bark extract and composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1. Preparation of Cinnamon Water Extract

[0113] Process flow of cinnamon water extract

[0114] Mix the cinnamon raw material and double-distilled water in a certain ratio (10g+100ml), extract with a rotary evaporator under vacuum, control the water temperature at 45°C, filter with qualitative filter paper after extraction for 6 hours, and filter out the residue , to obtain 50ml of cinnamon aqueous extract, and the resulting concentration is 0.2g / ml.

[0115] Physicochemical properties of cinnamon water extract

[0116] Cinnamon water extract is a mixture containing cinnamyl alcohol, cinnamic acid, cinnamaldehyde, eugenol and other organic compounds. The appearance is a yellow clear liquid with a pH of 4.6, slightly acidic, with a special fragrance and a light and slightly sweet taste.

[0117] HPLC spectrum

[0118] The HPLC collection of illustrative plates of cinnamon aqueous extract of the present invention is as figure 1 shown.

[0119] The ...

Embodiment 2

[0127] The preparation of embodiment 2 cinnamon-ethanol / water (10:90, v / v) extract

[0128] The ethanol extract of cinnamon was prepared according to the process of Example 1, except that the double distilled water was replaced by a mixed solvent system of ethanol / water (10:90, v / v), and the extraction temperature was 40°C. The dried extract is a yellowish powder, which can be dissolved in water to obtain a clear yellow liquid with a pH of 4.3.

[0129] The cinnamon water extract (indicated by CE) prepared in Example 1 was used in the following examples. In the experiment, 1 μl, 3 μl, and 6 μl of the extract of Example 1 were added to a 24-well cell culture plate, the cell culture medium was 1 ml, and the obtained concentrations were 0.2 mg / ml, 0.6 mg / ml, and 1.2 mg / ml, respectively.

Embodiment 3

[0130] Example 3. The induction of cinnamon water extract to the differentiation of 3T3-L1 cells into adipocytes

[0131] 3T3-L1 cells were seeded in 24-well plates, cultured for 2 days, and induced to differentiate with DM (10 μg / ml insulin, 1 μM dexamethasone, 0.5 mM cadenosine inhibitor), while adding different concentrations of CE (0.2 mg / ml , 0.6mg / ml). After 5 days, they were stained with Oil Red and observed under a microscope. Observation results such as figure 2 shown.

[0132] The results showed that CE could significantly promote the differentiation of 3T3-L1 cells into adipocytes. Since PPARγ is a sufficient and necessary condition for adipocyte differentiation, it is speculated that CE may stimulate the expression of PPARγ.

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Abstract

The invention relates to a preparation method of a cassia bark extract, the cassia bark extract prepared by the preparation method, a composition containing the extract and application of the extract in pharmacy or preparation of health care products. The preparation method comprises the following steps: a) uniformly mixing cassia bark raw material with a water-based solvent; b) extracting mixture obtained from the step a); and c) separating residue and finally getting the cassia bark extract. The cassia bark extract contains cinnamic alcohol, cinnamic acid, cinnamic aldehyde, eugenol and thelike, the appearance is yellow clear liquid, and the pH is about 4.6. The invention further provides the composition containing the extract. The cassia bark extract can be used for preparing medicaments or health care products with double effects of reducing blood glucose and reducing blood lipid and the function of protecting the liver.

Description

[0001] This application was submitted by the applicant on October 25, 2006. The application number is "200610117512.1", and the title of the invention is "the preparation method of cinnamon extract, cinnamon extract, its composition and use". Application. technical field [0002] The invention relates to a traditional Chinese medicine extract, its preparation method and composition and its use in the preparation of medicines or health care products. Specifically, the present invention relates to a preparation method of cinnamon extract, a cinnamon extract prepared by the method, a composition containing the extract, and the extract has dual effects of lowering blood sugar and blood fat and protecting Use in medicines for liver function. Background technique [0003] Diabetes is a very old disease, which is called "Xiao Ke" in traditional Chinese medicine, which means weight loss and polydipsia. Modern medicine believes that it is a common endocrine disease, which is caused...

Claims

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Application Information

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IPC IPC(8): A61K36/54A61P3/06A61P3/10
Inventor 秦莹盛小燕
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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