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Method for preparing composite probiotic spraying agent

A technology of compound probiotics and spraying agent, applied in the field of microorganisms, can solve problems such as difficulty in the production of compound microbial fertilizers, and achieve the effects of prolonging the viewing period, prolonging the green period of the lawn and improving the taste.

Inactive Publication Date: 2013-07-24
沈阳科丰牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the above problems, the present invention, based on the current research results of effective microorganisms related to biological fertilizers, develops a targeted production method for the compound combination of various functional microorganisms, and solves the problem of difficult production of compound microbial fertilizers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Value-added culture of compound strains: Candida utilis, Streptococcus lactis, Lactobacillus acidophilus, Bacillus subtilis, Bradyrhizobium soybean, Azospirillum adipogenicum, Rhodopseudomonas palustris, Spore Bacillus, colloid bacillus and azotobacter rufica were proliferated and cultured in their respective mediums at an inoculum amount of 3% by weight until the logarithmic growth phase.

[0029] 2) The fermented product of composite microorganisms: mix the Candida utilis, Streptococcus lactis, Lactobacillus acidophilus, and Bacillus subtilis that were respectively multiplied and cultured above in a volume ratio of 1:1:1:1, and then mix the strains Inoculate 4% by weight in the liquid fermentation medium, ferment and cultivate for 70 hours at 30°C, the pH value of the fermented product is 4.5-7.5, and the total amount of beneficial bacteria in each milliliter of medium reaches more than 3 billion, of which the liquid fermentation culture The base is 2% of glucose, ...

Embodiment 2

[0049] 1) Value-added culture of compound strains: Candida utilis, Streptococcus lactis, Lactobacillus acidophilus, Bacillus subtilis, Bradyrhizobium soybean, Azospirillum adipogenicum, Rhodopseudomonas palustris, Spore Bacillus, Bacillus colloidus and Azotobacter rufica were proliferated and cultured in their respective mediums at an inoculum amount of 1% by weight until the logarithmic growth phase.

[0050] 2) Fermented product of complex microorganisms: mix the above-mentioned Candida utilis, Streptococcus lactis, Lactobacillus acidophilus, and Bacillus subtilis that were propagated and cultivated separately, and then inoculate the mixed strains into the liquid fermentation culture according to 3% by weight Fermented and cultivated for 72 hours at 25°C, and the obtained fermented product was mixed with Bradyrhizobium soybean, Azospirillum adipogenicum, Rhodopseudomonas palustris, Bacillus megaterium, gum The fermented product of complex microorganisms obtained by mixing Ba...

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Abstract

The invention relates to the field of microbes, in particular to a method for preparing a composite probiotic spraying agent. The method comprises the following steps of: performing propagation culture in respective culture medium according to the inoculation quantity of 1 to 4 weight percent, then mixing, inoculating 3 to 4 weight percent of mixture into a liquid fermentation medium, and finallyperforming fermentation culture for 48 to 72 hours at the temperature of between 25 and 37 DEG C. The leaf surface spraying agent can prolong the growth period of vegetables and improve the yield andthe taste of the vegetables, and the yield of the vegetables can be improved by 5.5 to 10.0 percent; and the ornamental period of flowers can be prolonged by 8 to 10 days.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a preparation method of a compound probiotic spraying agent. Background technique [0002] Microbial foliar fertilizer is a kind of microbial fertilizer. It is a specific product containing live microorganisms that is sprayed on the surface of plants. Growth, improve the ability to resist diseases and insect pests. As people gradually discover the types of microorganisms that can be used as microbial fertilizers, various microorganisms such as nitrogen-fixing bacteria, bacillus, photosynthetic bacteria, cellulolytic bacteria, lactic acid bacteria, yeasts, and actinomycetes can be used to develop microbial fertilizers. Moreover, different microbial species have different growth-promoting mechanisms and functions as microbial fertilizers. Therefore, it is an effective solution to explore the use of complex bacterial flora as microbial fertilizers. [0003] However, in the production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 高林白子金白春生宋良敏高忠武王守峰于波陈思刘丽丽单立秋白志超朱天勇吴琼邵中金陈德民
Owner 沈阳科丰牧业科技有限公司
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