Unlock instant, AI-driven research and patent intelligence for your innovation.
Gossypium hirsutum mitogen-activated protein kinas 6 (GhMAPK6) gene and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A protein kinase gene, mitogen activation technology, applied in the field of molecular biology and biology
Inactive Publication Date: 2011-08-17
SHANDONG AGRICULTURAL UNIVERSITY
View PDF2 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0007] After searching, there is no report about the cotton yield-related gene GhMAPK6 in domestic and foreign literatures and patents
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment approach ( 1
[0137] Embodiment (1): A method for cloning cotton mitogen-activated proteinkinase gene GhMAPK6
[0138] 1. Extraction of RNA: Extraction of cotton total RNA using TRIZOL kit
[0139] 2. Synthesis of the first strand of cDNA (transgen company cDNA first strand synthesis kit method)
[0140] Add the following reagents to a 0.25ml centrifuge tube:
[0146] After mixing by pipetting, put in a water bath at 42°C for 30 minutes, then in a water bath at 85°C for 5 minutes, cool on ice for 5 minutes, centrifuge lightly, and store for later use.
[0240] Using the full-length sequencing plasmid of the gene as a template, the above-mentioned forward and reverse primers were used for PCR reaction.
[0241] (2) Take 4 μl of the PCR product and connect it to the pMD18-T Simple vector, and the operation steps are carried out according to the instructions of the pMD18-T Simple Vector product of TaKaRa Company. Then the ligation product was transformed into Escherichia coli DH5α competent cells, and cultured overnight on LB solid medium containing kanamycin (50mg / L). Pick the white colony Pick ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to a gossypium hirsutum mitogen-activated protein kinas 6 (GhMAPK6) gene and application thereof, belonging to the fields of molecular biology and biotechnology. The GhMAPK6 gene is obtained by adopting homologous cloning and RACE [Rapid-Amplification of cDNA (complementary Deoxyribonucleic Acid) Ends] technology. The cDNA sequence of the gene is put under a CaMV 35S promoter to construct a plantexpression vector, and nicotiana benthamiana is transformed to excessively express the gene in tobacco. An experiment proves that a transgenic tobacco plant has remarkably improved yield than a non-transgenic tobacco plant in the aspects of increase in the leaf area, increase in the tillering number, increase in the infruit number and increase in the ripening rate. Therefore, the gene has important realistic significance in improving the crop yield trait and increasing the crop yield by a gene engineering way according to the yield increasing property of the gene.
Description
(1) Technical field [0001] The invention relates to the cloning and recombination of a cotton mitogen-activated proteinkinase gene GhMAPK6 and its application in improving the yield of transgenic tobacco, belonging to the fields of molecular biology and biotechnology. (2) Background technology [0002] As the world population continues to surge, people's demand for food is also increasing dramatically. At the same time, the wanton development of resources and environment by human beings has gradually destroyed the ecological balance and greatly affected the food production. In our country, with the continuous improvement of economic level and the continuous acceleration of urbanization and industrialization, the proportion of agricultural population and usablearable land is decreasing year by year. Therefore, how to increase food production to meet people's growing basic living material needs is the current Problems to be solved. [0003] The traditional methods to incre...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
