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Preparation method of ivy saponins ingredient and application of the ingredient to resisting tumors

A technology of hedera saponins and anti-tumor drugs, which is applied in the preparation of hedera saponins and its anti-tumor applications, and can solve the problems of lack of experimental evidence for the treatment of lung cancer, lack of experimental evidence, and no obvious anti-cancer activity

Active Publication Date: 2011-09-14
JIANGXI HERBFINE HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] CN 1308000C also lacks experimental basis for treating other solid tumors other than lung cancer
[0008] In addition, BTW58 components and compound I have been proved to have no obvious anticancer activity against human renal carcinoma, human nasopharyngeal carcinoma, etc.
[0009] CN 1308000C speculates that the experimental data of anti-lung cancer can resist all solid tumors, and there is no experimental basis

Method used

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  • Preparation method of ivy saponins ingredient and application of the ingredient to resisting tumors
  • Preparation method of ivy saponins ingredient and application of the ingredient to resisting tumors
  • Preparation method of ivy saponins ingredient and application of the ingredient to resisting tumors

Examples

Experimental program
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Effect test

Embodiment example 1

[0035] The first step is to grind Pulsatilla pulsatillae into coarse powder, soak in water for 0.5-2h, heat and reflux extract 6-12 times the amount for 2-3 times, each time for 1-3h, filter, and combine the filtrates. The above-mentioned filtrate was concentrated under reduced pressure at 50-70°C to 0.5-1.0g raw medicinal materials / mL, refrigerated overnight at 0-4°C, separated from the gel, and filtered to obtain Pulsatilla pulsatilla extract.

[0036] In the second step, add sodium hydroxide solution to the aqueous solution of Pulsatillae extract to adjust the pH to 10-13, heat and boil for 4-10 hours, measure the pH every 30-60 minutes, keep it at pH 10-13, let it cool, add hydrochloric acid solution, and adjust the pH 3-7, centrifuge, remove the precipitate, and obtain the hydrolyzate of Pulsatilla radix extract

[0037] In the third step, the hydrolyzate of Pulsatillae extract is adsorbed by D101 or AB-8 or ADS-17 macroporous resin, eluted with water, 30-40% ethanol, 50-...

Embodiment example 2

[0046] The first step is to grind Pulsatilla pulsatillae into coarse powder, soak in 10-45% ethanol for 0.5-2h, heat and reflux extract 6-12 times the amount for 2-3 times, each time for 1-3h, filter, and combine the filtrates. The above-mentioned filtrate was concentrated under reduced pressure at 50-70°C to 0.5-1.0g raw medicinal materials / mL, without alcohol smell, refrigerated overnight at 0-4°C, gel separated, filtered, and Pulsatilla pulsatilla extract was obtained.

[0047] In the second step, add sodium hydroxide solution to the aqueous solution of Pulsatillae extract to adjust the pH to 10-13, heat and boil for 4-10 hours, measure the pH every 30-60 minutes, keep it at pH 10-13, let it cool, add hydrochloric acid solution, and adjust the pH 3-7, centrifuge, remove the precipitate, and obtain the hydrolyzate of Pulsatilla radix extract

[0048] In the third step, the hydrolyzate of Pulsatillae extract is adsorbed by D101 or AB-8 or ADS-17 macroporous resin, eluted with...

Embodiment example 3

[0057] The first step is to grind Pulsatilla pulsatillae into coarse powder, soak in 45-95% ethanol for 0.5-2h, heat reflux to extract 6-12 times the amount for 2-3 times, each time for 1-3h, filter, and combine the filtrates. The above-mentioned filtrate was concentrated under reduced pressure at 50-70°C to 0.5-1.0g raw medicinal materials / mL, without alcohol smell, refrigerated overnight at 0-4°C, gel separated, filtered, and Pulsatilla pulsatilla extract was obtained.

[0058] In the second step, add sodium hydroxide solution to the aqueous solution of Pulsatillae extract to adjust the pH to 10-13, heat and boil for 4-10 hours, measure the pH every 30-60 minutes, keep it at pH 10-13, let it cool, add hydrochloric acid solution, and adjust the pH 3-7, centrifuge, remove the precipitate, and obtain the hydrolyzate of Pulsatilla radix extract

[0059] In the third step, the hydrolyzate of Pulsatillae extract is adsorbed by D101 or AB-8 or ADS-17 macroporous resin, eluted with ...

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Abstract

This invention relates to a preparation method of an ivy saponins ingredient 3-O-Alpha-L-rhamnopyranosyl-(1-2) (Beta-D-glucopyranosyl-(1-4))-Alpha-L-pyridine Nan ivy saponins arabinose and application of the ingredient to resisting tumors. The invention also relates to application of an extract, which is derived from Pulsatillachinensis (Bunge) Regel rhizome and contains the compound, to resisting the tumors above. The tumors include liver cancer, stomach cancer, glioma, breast cancer, cervical cancer, leukemia, colon cancer, lymphoma and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine and veterinary medicine, and relates to a hedera saponin component 3-O-α-L-rhamnopyranose-(1→2)[β-D-glucopyranose-(1→4) ]-α-L-Arabinopyranosyl hedera saponin preparation method and its anti-tumor application, such as liver cancer, gastric cancer, glioma, breast cancer, cervical cancer, leukemia, colon cancer, lymphoma, etc. . [0002] The present invention also relates to the use of an extract containing the compound from the rhizome of Pulsatilla chinensis (Bunge) Regel as an anti-tumor agent as described above. Background technique [0003] The medicinal material of Pulsatilla is taken from the dried root of Pulsatilla genus Pulsatilla, which is bitter in taste and cold in nature. Return stomach, large intestine meridian, tool heat-clearing and toxic substances removing, the merit of cooling blood and stopping dysentery. For pyretotoxic blood dysentery, pruritus vulvae leukorrhagia, amoebic ...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P35/00C07J63/00
Inventor 杨世林许琼明简晖刘艳丽陈兰英冯育林罗晓健李笑然罗颖颖李俊
Owner JIANGXI HERBFINE HI TECH
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