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Rotor-type internal-compression oil-gas mixed transport pump unit

A diketone and diene technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of few reports worldwide

Inactive Publication Date: 2011-10-05
ZHEJIANG XIANJU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] At present, there are not many reports on the method of converting intermediate A to intermediate B by microorganisms in the world, and the feeding concentration is as high as 7%.

Method used

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  • Rotor-type internal-compression oil-gas mixed transport pump unit
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  • Rotor-type internal-compression oil-gas mixed transport pump unit

Examples

Experimental program
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preparation example Construction

[0041] The preparation method of the steroid compound intermediate 11,17α-substituted pregna-1,4-diene-3,20-dione of the present invention uses microorganisms to prepare the 11,17α-substituted pregna-4- Conversion of ene-3,20-dione (intermediate A) to 11,17α-substituted pregna-1,4-diene-3,20-dione (intermediate B); the microorganism can adopt Arthrobacter simplex ( Arthrobacter Simplex, ATCC 6946 ); including the following steps:

[0042] The first step is to configure and process the culture medium;

[0043] The culture medium is configured according to the following proportions:

[0044] The culture medium includes nitrogen-containing substances, inorganic salts, water, 0.5-1.5% glucose (percentage by weight), and 0.5-1.5% corn steep liquor (percentage by weight);

[0045] Nitrogen-containing substances can be added with bean cake powder, peptone, yeast extract, yeast extract, beef extract, etc.; the addition amount is 0.2-0.5% (weight percentage);

[0046] Inorganic sal...

Embodiment 1

[0066] A 500ml flask was filled with 200ml of medium containing 1% (weight percent) glucose, 1% (weight percent) corn steep liquor, 0.3% (weight percent) peptone, 0.15% (weight percent) KH 2 PO 4 ; Sterilize the culture medium in an autoclave at 120°C for 30 minutes; cool it to 30±1°C, then inoculate the slant culture of Arthrobacter simplex in the culture medium; place the culture medium inoculated with the strain in a shaker Cultivate at 165rpm at 30±1°C for 24 hours;

[0067] 10.0 g of 11α, 17α-dihydroxy-pregn-4-ene-3,20-dione was pulverized, sterilized by dry heat, and then put into the culture medium; continued to stir and ventilate; control the pH to 5.4; keep the fermentation temperature at 30±1℃;

[0068] After 48 hours, the culture solution was harvested, and the solid matter was filtered and dried;

[0069] Decolorization and purification by conventional methods gave 6.21 g of 11α, 17α-dihydroxy-pregna-1,4-diene-3,20-dione, with a yield of 62.1% and a content of 9...

Embodiment 2

[0072] A 500ml flask was filled with 200ml of medium containing 1% (weight percent) glucose, 1% (weight percent) corn steep liquor, 0.3% (weight percent) peptone, 0.15% (weight percent) KH 2 PO 4 ; Sterilize the culture medium in an autoclave at 120°C for 30 minutes; cool it to 30±1°C, then inoculate the slant culture of Arthrobacter simplex in the culture medium; place the culture medium inoculated with the strain in a shaker Cultivate at 165rpm at 30±1°C for 24 hours;

[0073] 10.0 g of 11α, 17α-dihydroxy-pregn-4-ene-3,20-dione was pulverized, sterilized by dry heat, and then put into the culture medium; continued to stir and ventilate; control the pH to 7.8; keep the fermentation temperature at 30±1℃;

[0074] After 48 hours, the culture solution was harvested, and the solid matter was filtered and dried;

[0075] Decolorization and purification by conventional methods gave 6.32 g of 11α, 17α-dihydroxy-pregna-1,4-diene-3,20-dione, with a yield of 63.2% and a content of 9...

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Abstract

The invention relates to a rotor-type internal-compression oil-gas mixed transport pump unit, and belongs to the technical field of fluid transportation devices. The unit comprises a housing which supports a pair of conjugated rotors engaged with each other; the cavity in the housing is partitioned by the conjugated rotors into an inhalation chamber and a discharging chamber; the inhalation chamber is equipped with a fluid inlet, and the discharging chamber is equipped with a fluid outlet; the discharging chamber is partitioned into an engaging area and a discharging area by an isolating wall which is disposed between the conjugated rotors and the fluid outlet; at least one one-way valve is disposed at the isolating wall; when the one-way valve is closed, the engaging chamber is completely isolated from the discharging chamber are; when the one-way valve is opened, the engaging chamber is communicated with the discharging chamber. Even in a multiphase medium transporting process with an unstable gas-liquid ratio, the rotor-type internal-compression oil-gas mixed transport pump unit of the invention has the unique function of internally compressing the gas medium, pressurizing the gas to a discharging pressure so as to discharge the gas out of the pump; the invention can completely eliminate the gas blocking phenomenon, and can reliably realize the internal-compression transporting process of the transported multiphase medium.

Description

technical field [0001] The invention relates to a preparation method of a steroid compound, in particular to a preparation method of a steroid compound intermediate 11,17α-substituted pregna-1,4-diene-3,20-dione. Background technique [0002] 11,17α-substituted pregna-1,4-diene-3,20-dione (intermediate B) is a very important intermediate in steroids. A variety of corticosteroids can be prepared by using intermediate B as a raw material, especially in the field of steroid hormone production with phytosterols as starting materials in the future, and it is a necessary intermediate in the production process of various corticosteroids. These corticosteroids can treat a variety of human diseases, and are of great significance to the protection of people's health. [0003] The chemical formula of intermediate B is as follows: [0004] [0005] in [0006] R 1 Represents H, OH (including α or β position), O (forms ketone group with 11 C) [0007] R 2 stands for OH or acetat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12R1/06
Inventor 李龙王建应明华朱秀燕
Owner ZHEJIANG XIANJU PHARMA
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