Biological preservative for citrus based on antagonistic activity of rhodosporidium paludigenum fell&tallman

A marine bio-preservation and bio-preservation technology, applied in the fields of fruit and vegetable preservation, food preservation, food science, etc., can solve the problems of increasing the cost of bio-preservative preservatives, and achieve outstanding disease control effects, reduce costs, and ensure food safety.

Inactive Publication Date: 2011-11-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] And as we all know, the price of chitosan is much higher than that of calcium chloride (about 60 times that of calcium chloride), and the use of chitosan in biological preservation will inevitably increase the cost of biological preservation

Method used

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  • Biological preservative for citrus based on antagonistic activity of rhodosporidium paludigenum fell&tallman

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The preparation method of Rhodosporidium yeast suspension is as follows:

[0024] The following NYDA medium used is: beef extract 8g, yeast powder 5g, glucose 10g, agar 20g, water to 1000ml, high pressure (1kg / cm 2 ) sterilized at 121°C for 30 minutes.

[0025] NYDB seed culture medium is: beef extract 8g, yeast powder 5g, glucose 10g, water to 1000ml, high pressure (1kg / cm 2 ) sterilized at 121°C for 30 minutes.

[0026] Rhodosporidium IMI 394084 was maintained in NYDA medium at low temperature (4°C). The training program is as follows:

[0027] ①. Activation: Rhodosporidium IMI 394084 was first cultured in NYDA medium at 28°C for 48 hours, and then subcultured twice (each time in NYDA medium at 28°C for 48 hours);

[0028]The activated yeast obtained by repeating the subculture twice above was transferred to the NYDB seed medium with an inoculation loop, and cultivated at 28°C for 24 hours; The yeast was again connected to the NYDB seed medium with an inoculation...

Embodiment 1

[0032] Embodiment 1, biological preservative

[0033] At a concentration of 5 x 10 7 1 cell / ml, a volume of 2 liters of Rhodosporidium yeast IMI 394084 suspension, slowly add concentration of 2 liters of calcium chloride solution of 10% (w / v), after the calcium chloride solution has been added, continue to stir and mix for 5 Minutes, add water to dilute to 10 liters for later use. Contain 1×10 10 Rhodosporidium IMI394084 cells, 20g calcium chloride, and the rest are water.

Embodiment 2

[0034] Embodiment 2, biological preservative

[0035] Change the consumption of the calcium chloride solution in embodiment 1 into 1.95 liters, all the other are with embodiment 1. Contain 1×10 10 Rhodosporidium IMI 394084 cells, 19.5g calcium chloride, and the rest are water.

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Abstract

The invention discloses a biological preservative for citrus based on antagonistic activity of rhodosporidium paludigenum fell&tallman. The preservative is characterized in that the preservative comprises rhodosporidium paludigenum fell&tallman suspension, calcium chloride and water; every lL of preservative contains 1*10<10>-1*10<12> rhodosporidium paludigenum fell&tallman cells, 19.5-20.5g of calcium chloride and the balance of water; and rhodosporidium paludigenum fell&tallman is rhodosporidium paludigenum fell&tallman with the collection number of IMI394084. The biological preservative can effectively control the postharvest diseases of the citrus fruits, without using chemical bactericides.

Description

technical field [0001] The invention relates to the technical field of prevention and treatment of post-harvest fruit diseases, and more specifically relates to a biological antiseptic and fresh-keeping technology for preventing and curing citrus fruit diseases. Background technique [0002] Citrus is one of the most productive fruits and has a long history of cultivation. However, citrus production areas are generally located in subtropical regions, and the temperature and humidity are more suitable for the growth of fruit pathogens, and the postharvest losses caused by pathological rot are huge every year. At present, chemical fungicides are the main and most effective method to control postharvest fruit rot. However, with the development of the country's economy and the improvement of consumption levels, people's awareness of food safety and environmental protection has increased, and the scope of use of chemical fungicides has become more and more restricted. In additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155A23B7/157
Inventor 余挺郑晓冬努尔哈音朱瑞瑜郭峻路来风卢黄娉
Owner ZHEJIANG UNIV
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