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Tangerine biological antistaling agent based on activity of rhodosporidium and fruit elicitor

A technology of Rhodosporidium yeast and biological preservative, which is applied in the field of biological antiseptic and fresh-keeping for the prevention and treatment of citrus fruit diseases. and the effect of development

Inactive Publication Date: 2013-01-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, salicylic acid is one of the most widely studied elicitors, but there are few studies on the effects of cytokinin, auxin, gibberellin and other plant hormones on fruit resistance.

Method used

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  • Tangerine biological antistaling agent based on activity of rhodosporidium and fruit elicitor
  • Tangerine biological antistaling agent based on activity of rhodosporidium and fruit elicitor

Examples

Experimental program
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Effect test

preparation example Construction

[0031] The preparation method of Rhodosporidium yeast suspension is as follows:

[0032] The following NYDA medium used is: beef extract 8g, yeast powder 5g, glucose 10g, agar 20g, water to 1000ml, high pressure (1kg / cm 2 ) sterilized at 121°C for 30 minutes. The used NYDB seed culture medium is: beef extract 8g, yeast powder 5g, glucose 10g, water is fixed to 1000ml, high pressure (1kg / cm 2 ) sterilized at 121°C for 30 minutes.

[0033] Rhodosporidium IMI 394084 was maintained in NYDA medium at low temperature (4°C). The training program is as follows:

[0034] ①. Activation: Rhodosporidium IMI 394084 was first cultured in NYDA medium at 28°C for 48 hours, and then subcultured twice (each time in NYDA medium at 28°C for 48 hours);

[0035] The activated yeast obtained by repeating the subculture twice above was transferred to the NYDB seed medium with an inoculation loop, and cultivated at 28°C for 24 hours; The yeast was again connected to the NYDB seed medium with an i...

Embodiment 1

[0039] Embodiment 1, a kind of citrus biological preservative based on rhodosporidium and fruit elicitor activity

[0040] In 2 liters of Rhodosporidium IMI 394084 suspension (effective concentration is 5×10 7 cells / ml), slowly add 1000 ml of SA solution with a concentration of 1000 mg / L, 1000 ml of IBA solution with a concentration of 1000 mg / L and 1000 ml of gibberellin solution with a concentration of 1000 mg / L, stir and mix for 5 minutes, and dilute with water to 10 liters for spare. Each L of the final biological preservative contains 1×10 10 Rhodosporidium IMI 394084 cells, SA100mg / L, IBA100mg / L and gibberellin 100mg / L, the rest is water.

Embodiment 2

[0041] Embodiment 2, a kind of citrus biological preservative based on rhodosporidium and fruit elicitor activity

[0042] In 2 liters of Rhodosporidium IMI 394084 suspension (effective concentration is 5×10 7 1000 milliliters of SA solution with a concentration of 1000 mg / L, 100 milliliters of an IBA solution with a concentration of 1000 mg / L and 1000 milliliters of a kinetin solution with a concentration of 1000 mg / L were slowly added in the concentration of 1000 mg / L), stirred and mixed for 5 minutes, and diluted with water to 10 liters spare. Each L of the final biological preservative contains 1×10 10 Rhodosporidium IMI 394084 cells, SA100mg / L, IBA10mg / L and kinetin 100mg / L, the rest is water.

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Abstract

The invention discloses a tangerine biological antistaling agent based on the activity of rhodosporidium and a fruit elicitor, which consists of rhodosporidium suspension, gibberellin / kinetin, salicylic acid, auxin and water, wherein each 1 liter of antistaling agent contains 1*1010 to 1*1012 rhodosporidium cells, 50 to 150 milligrams of gibberellin or kinetin, 8 to 100 milligrams of salicylic acid, 10 to 500 milligrams of auxin and the balance of water; and the rhodosporidium is rhodosporidium paludiagenum Fell and Tallman of which the collection number is IMI 394084. The biological antistaling agent can control post-harvest diseases of tangerine fruits effectively on the premise that chemical bactericides are not used.

Description

technical field [0001] The invention relates to the technical field of prevention and treatment of post-harvest fruit diseases, and more specifically relates to a biological antiseptic and fresh-keeping technology for preventing and curing citrus fruit diseases. Background technique [0002] Citrus has a long history of cultivation and is one of the important fruits. However, citrus production areas are generally located in subtropical regions, and the temperature and humidity are more suitable for the growth of fruit pathogens, and the postharvest losses caused by pathological rot are huge every year. At present, chemical fungicides are the main and most effective method to control postharvest fruit rot. However, with the development of the country's economy and the improvement of consumption levels, people's awareness of food safety and environmental protection has increased, and the scope of use of chemical fungicides has become more and more restricted. In addition, du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/155
Inventor 余挺郑晓冬朱瑞瑜努尔哈音蓝天演钱成济
Owner ZHEJIANG UNIV
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