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Ionic liquid- polyacrylamide gel, preparation method and purpose thereof

A polyacrylamide gel and ionic liquid technology, which is applied in the field of protein separation and analysis, can solve the problem of low protein separation

Inactive Publication Date: 2013-06-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For the defect that the SDS-PAGE method has a low degree of separation of different proteins with equal or similar molecular weights, one of the purposes of the present invention is to provide an ionic liquid-polyacrylamide gel; specifically, the ionic liquid-polyacrylamide The ionic liquid in the gel is an ionic liquid with an alkyl carbon chain carbon atom number ≤ 4 on the cation

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  • Ionic liquid- polyacrylamide gel, preparation method and purpose thereof
  • Ionic liquid- polyacrylamide gel, preparation method and purpose thereof
  • Ionic liquid- polyacrylamide gel, preparation method and purpose thereof

Examples

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Embodiment 1

[0072] The mass concentration of 1-ethyl-3-methylimidazolium tetrafluoroborate prepared respectively is 0.05% (w / v), 0.1% (w / v), 0.2% (w / v), 0.3% (w / v ), 0.6% (w / v) and 1.0% (w / v) of 1-ethyl-3-methylimidazolium tetrafluoroborate-polyacrylamide gel ([C 2 mim] BF 4 -PAG) and 1-butyl-3-methylimidazolium tetrafluoroborate mass concentrations are 0.05% (w / v), 0.1% (w / v), 0.2% (w / v), 0.3% (w / v), 0.6% (w / v) and 1.0% (w / v) 1-butyl-3-methylimidazolium tetrafluoroborate-polyacrylamide gel ([C 4 mim] BF 4 -PAG), use said gel to carry out ionic liquid-sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation to protein molecular weight Marker respectively. method such as figure 1 shown.

[0073] The preparation method and electrophoresis method of the gel are as follows:

[0074] (1) Preparation of ionic liquid-polyacrylamide gel

[0075] The mass concentration of 1-ethyl-3-methylimidazolium tetrafluoroborate prepared respectively is 0.05% (w / v), 0.1% (w / v), 0.2% (w / v),...

Embodiment 2

[0088] The brominated N-butyl-3-methylpyridine bromide mass concentration is 0.05% (w / v), 0.3% (w / v) and 1.0% (w / v) respectively picoline-polyacrylamide gel ([C 4 mpd]Br-PAG), brominated N-butyl-3-methylpyrrolidine mass concentration is 0.05% (w / v), 0.3% (w / v) and 1.0% (w / v) brominated N -Butyl-3-methylpyrrolidine-polyacrylamide gel ([C 4 mprd]Br-PAG) and 1-butyl-3-methylimidazole bromide with a mass concentration of 0.05% (w / v), 0.3% (w / v) and 1.0% (w / v) of brominated 1- Butyl-3-methylimidazole-polyacrylamide gel ([C 4 mim] Br-PAG), using said gel to carry out ionic liquid-sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation to ten-fold diluted human serum samples respectively, the method is as follows figure 1 shown.

[0089] (1) Preparation of ionic liquid-polyacrylamide gel

[0090] Separately prepare [C 4 mpd] Br mass concentrations of 0.05% (w / v), 0.3% (w / v) and 1.0% (w / v) 4 mpd] Br-PAG, [C 4 mprd] Br mass concentration of 0.05% (w / v), 0.3% (w / v) ...

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Abstract

The invention relates to an ionic liquid-polyacrylamide gel, a preparation method and a purpose thereof, and belongs to the technical field of protein separation and analysis. The ionic liquid-polyacrylamide gel comprises a monomer, a cross-linking agent, a gel buffer system, a catalyst, an accelerator and an ionic liquid, wherein the ionic liquid has cations having no more than 4 carbon atoms onan alkyl carbon chain, and has a mass concentration of 0.05-1.0%(w / v). The ionic liquid, the monomer, the cross-linking agent and the gel buffer system are mixed together, and added with the catalystand the accelerator to be mixed uniformly; and the mixture is solidified to obtain the ionic liquid-polyacrylamide gel. The ionic liquid-polyacrylamide gel can be applied to protein electrophoretic separation, namely, an ionic liquid-sodium dodecyl sulfate-polyacrylamide gel is used for electrophoresis separation of an SDS treated protein. Especially, for different proteins having a same or a similar molecular weight, a rapid, simple, green and efficient separation thereof can be realized.

Description

technical field [0001] The present invention relates to a kind of ionic liquid-polyacrylamide gel (ILs-PAG), its preparation method and application, specifically, the ionic liquid (ILs) in the described ionic liquid-polyacrylamide gel is cationic upper alkyl The invention relates to an ionic liquid with a base carbon chain carbon atom number less than or equal to 4, belonging to the technical field of protein separation and analysis. Background technique [0002] Since the completion of the sequencing of the human genome, proteomics research has received extensive attention. In recent years, proteomics research has been widely used in basic life sciences, medical analysis, and disease marker research. Complete proteomics analysis includes four parts: sample preparation, protein separation, protein analysis identification and characterization, among which protein separation and analysis technology is the key to proteomics research, which restricts the development of proteomi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/56C08J3/075G01N27/447
Inventor 屈锋张涛盖青青
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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