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Plant salt resistant associated protein AtST1, coded genes and application thereof

A coding gene and plant technology, applied in the field of plant salt tolerance-related protein AtST1 and its coding gene and application, can solve the problems of unclear gene function and achieve the effect of improving hyperosmotic resistance and ABA resistance

Active Publication Date: 2013-09-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The genome size of Arabidopsis thaliana is 125Mbp, with about 27,000 genes, and the functions of many genes are still not very clear

Method used

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  • Plant salt resistant associated protein AtST1, coded genes and application thereof
  • Plant salt resistant associated protein AtST1, coded genes and application thereof
  • Plant salt resistant associated protein AtST1, coded genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Acquisition of proteins and genes encoding them in response to high-salt stress in plants

[0043] Total RNA was extracted from Arabidopsis thaliana (Columbia type, also known as Col-0 ecotype) seedlings (purchased from the Arabidopsis Biological Resource Center (ABRC)) seedlings for 10 days, and the RNA was digested with DNase from Takara Company. The digested DNA was reverse-transcribed to obtain cDNA. Using the cDNA as a template, a pair of primers respectively provided with XbaI and KpnI restriction sites were used for PCR amplification to obtain the full-length cDNA of salt-tolerance-related genes. The primer sequences are as follows:

[0044] Primer1: 5'-tctaga ATGGAATCGAGTAGCGTTGATGAG-3' (XbaI);

[0045] Primer2: 5'-ggtaccTTACGAGGCGTGAAAGATGCGTTGC-3' (KpnI).

[0046] The high-fidelity Pyrobest DNA polymerase from Takara Company was used for amplification. The amplification system was 50 μL, containing 5.0 μL of 10×Pyrobest PCR buffer, 1.0 μL of 10 mM ...

Embodiment 2

[0053] Example 2. Functional verification of proteins and genes encoding them in response to high-salt stress in plants

[0054] 1. Obtaining AtST1 overexpression plants (Super: AtST1-1 and Super: AtST1-2)

[0055] 1. Intermediate vector pCAMBIA1300: Construction of Super

[0056] Utilize primers aagcttGTGGGCCTGTGGTCTCAAGAT and tctagaCTAGAGTCGATTTGGT from plasmid pMSP-1 (GenBank number is EU181145) to amplify the Super promoter of the Super promoter whose nucleotide sequence is shown in the 113-1112 position from the 5' end of sequence 3 in the sequence listing Sequence; the amplified Super promoter was double-digested with HindIII and XbaI, and the Super promoter fragment was recovered and inserted between the HindIII and XbaI restriction sites of plasmid pCAMBIA1300 (GenBank No. FJ362601) to obtain pCAMBIA1300: Super plasmid.

[0057] 2. Super: Construction of AtST1

[0058] The recombinant vector pMD18-AtST1 containing the AtST1 gene obtained in Example 1 and the interme...

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Abstract

The invention discloses a plant salt resistant associated protein AtST1, its coded genes and application. The plant salt resistant associated protein provided by the invention is named as AtST1, is protein represented by the following 1) or 2): 1) protein composed of an amino acid sequence of the SEQ ID No.2 in the sequence list; and 2) protein derived from 1) by replacing and / or deleting and / or adding one or more amino acid residues in the SEQ ID No.2 in the sequence list and associated with plant salt resistance. The coded gene AtST1 of the protein provided by the invention is expressed by the induction of high salt. The high salt resistance, high permeability resistance and ABA resistance of the transgenic plant obtained by inducing the gene AtST1 in wild arabidopis thaliana are improved. The salt resistant gene disclosed in the invention has important theoretical and practical significances for studying plant high salt resistant molecule mechanism and breeding plant high salt resistant varieties.

Description

technical field [0001] The invention relates to a plant salt tolerance-related protein, its coding gene and application. Background technique [0002] The food problem is one of the major problems facing the world today. Soil salinization is an important limiting factor limiting agricultural production. Saline soil is a worldwide low-yielding soil, even barren. Therefore, understanding the response mechanism of plants to high-salt stress and improving the tolerance of plants to high-salt stress has become an important research work on how to further increase crop yield, and has attracted much attention from governments and plant and agricultural scientists around the world. [0003] In the long-term evolutionary process, higher plants have gradually evolved a set of systems for sensing and transmitting high-salt signals, and formed a series of physiological or developmental mechanisms to respond to high-salt stress in the environment and minimize the effects of high-salt s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415
Inventor 陈益芳武维华冯翠珠
Owner CHINA AGRI UNIV
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