Surface plasmon resonance sensing detection system and detection method thereof

Abstract

The invention is applicable to the technical field of photoelectric detection, and provides a surface plasmon resonance sensing detection system and a detection method thereof; the surface plasmon resonance sensing detection system comprises a light source, a first optical splitter, a first reflector, a prism, a second reflector, a polarizer and a controller. In the invention, a Michelson interferometer structure is combined with a scanning mechanism, phase changing information is reflected by the separation distance of light intensity envelope maximum of P polarized light; and then the changing information of a sample refractive index is further obtained, so that fussy phase extracting algorithms are avoided, and the practicability is increased. The Michelson interferometer structure is composed of a light source, a first optical splitter, a first reflector, a second reflector and a first detector, and the controller is used for controlling a stepping motor or a servo motor to drive the first reflector to perform reciprocating motion so as to form the scanning mechanism; therefore, the surface plasmon resonance sensing detection system is simple in structure and low in cost.

Description

technical field [0001] The invention belongs to the technical field of photoelectric detection, and in particular relates to a surface plasmon resonance sensing detection system and a detection method thereof. Background technique [0002] Surface Plasmon Resonance (SPR) is an emerging sensing technology, which has the advantages of high sensitivity, high throughput, easy to achieve specific detection and real-time performance, and does not require labeling. It has been widely used in biological , medicine, food quality and safety, chemical and environmental monitoring and other industries, especially the online real-time detection of biomolecules such as DNA and protein, protein molecules and drug-protein, nucleic acid-nucleic acid, antigen-antibody, receptor-ligand, etc. interactions among others. [0003] At present, SPR sensing technologies mainly include angle type, spectral type and phase type. Among them, phase type SPR has higher sensitivity and has obvious advantag...

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Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a surface plasmon resonance sensing detection system, which aims to solve the problem of difficult phase resolution of the existing phase-type surface plasmon resonance sensing technology

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