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Methods for culturing and preserving syntrichia caninervis mitt. protonemata

A technology of erythritis dentatus and cultivation methods, applied in the direction of plant preservation, horticultural methods, botanical equipment and methods, etc., can solve the problems of slow propagation speed of desert mosses, increase the coverage of surface biological crusts, reduce Surface erosion, the effect of enhancing the stability of the sand surface

Inactive Publication Date: 2013-03-27
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

[0008] Aiming at the technical status quo of biotechnology cultivation and preservation of Erythrodonticum at home and abroad, the purpose of the present invention is to provide a method for cultivating and preserving the protonema of Erythrodontoides that can be used for desertification control in arid areas, which is beneficial to A large number of artificial multiplication of erythrosa solves the technical problem of the slow natural reproduction speed of desert moss, and provides feasible biological materials for desert biological crust and windbreak and sand fixation

Method used

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  • Methods for culturing and preserving syntrichia caninervis mitt. protonemata

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Embodiment 1

[0026] Embodiment 1: the culture method of erythrosa dentifera protonema

[0027] The specific training steps include:

[0028](1) Material selection and processing: In the Gurbantunggut Desert, select the well-developed moss crust plaques with Erythrosis dentatus as the construction species, and select some robust Erythrosis dentifera plants; first use tap water Rinse well, and then rinse repeatedly with sterile distilled water; soak and disinfect the cleaned moss plants in 70% alcohol for 20 seconds on the sterile operating table, take them out and rinse them with sterile distilled water for 3-4 times; clean the cleaned moss plants Put it on a sterile operating table and use sterile air to dry naturally; in a sterilized mortar, slightly grind the air-dried moss plants into pieces for later use.

[0029] (2) Inoculation and cultivation: the moss fragments obtained in the above steps were diluted with sterile distilled water, and then inoculated in a container containing BG ...

Embodiment 2

[0033] Embodiment Two: Preservation method of protonema of dentate rib erythritis

[0034] The specific training steps include:

[0035] (1) Place the harvested moss protonema in a cool and ventilated place to air dry.

[0036] (2) The air-dried moss protonema is placed in a sterile sealed plastic bag and stored in a -40°C refrigerator for later use. The photosynthetic activity is represented by the fluorescence value (Fv / Fm) of chlorophyll a, and the concentration measured at room temperature is 2g. L -1 The fluorescence value (Fv / Fm) of the protonema was 0.36±0.01, and its activity was significantly higher than that of the protonema preserved at other temperatures.

[0037] Adopting the method for cultivating and preserving the protonema of E. dentatus provided by the present invention, it is expected that a large amount of artificial multiplication of E. dentata will be carried out, and the artificial rapid cultivation of desert moss and its biological crusts will be real...

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Abstract

The invention discloses methods for culturing and preserving syntrichia caninervis mitt. protonemata. The method for culturing syntrichia caninervis mitt. protonemata comprises the following steps: firstly selecting proper materials, carrying out washing, sterilizing, washing, air-drying, grinding and other treatments on the proper materials, then inoculating moss pieces to a BG11 solid culture medium, carrying out induction culture on the moss pieces in an illuminating incubator under the conditions that the temperature is 22 DEG C, the illumination intensity is 27mu mol.m<-2>.s<-1> and the light / dark time is 14h / 10h until the protonemata appear and then transferring the protonemata to liquid culture for progressive expanding propagation. The method for preserving syntrichia caninervis mitt. protonemata is characterized in that after being collected, the protonemata are naturally air-dried, are filled in sealed plastic bags and then are preserved at - 80 DEG C. The methods are beneficial to mass artificial expanding propagation of syntrichia caninervis mitt.; the protonemata can be developed into new plants, which provides a new way for artificial expanding propagation of syntrichia caninervis mitt.; and the methods have broad application fields.

Description

field of invention [0001] The invention relates to the biotechnical field of cultivation and preservation of desert moss, in particular, the invention relates to the technical field of cultivation and preservation of erythroma denticola. Background technique [0002] In recent years, my country's desertification has become more and more serious, and it is one of the countries with the most serious desertification in the world. The total area of ​​desertified land has reached 3.73 million square kilometers, and the annual expansion rate is 2460 square kilometers. The annual direct economic loss due to desertification is as high as 540 million billion. In view of this serious situation, it is urgent to find an effective way to prevent the desertification of land resources. [0003] Traditional windbreak and sand fixation measures such as afforestation, grass grid sand barriers and clay sand barriers are effective on the surface, but they cannot fundamentally solve the problem ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01N3/00
Inventor 张元明张丙昌王敬竹
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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