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Method for purifying and separating Microcoleus vaginatus

A technology for the purification and separation of Microcoletheca, which is applied in the field of purification and separation of Microcoletheca, the organisms cultivated and preserved in desert moss, can solve the problems that have not been reported, and achieve strong operability and increase coverage , The effect of the method is simple and easy

Inactive Publication Date: 2012-09-19
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] After searching, the existing research results show that there is no report on the purification and separation biotechnology of Microcoletheca at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Example: Purification and Separation of Microcodyle

[0025] Specific training steps include:

[0026] (1) Sampling: Prepare a PVC pipe with a diameter of 20 cm and a height of 10 cm. Take the well-developed algal crust in the desert environment, use the PVC pipe to sample, seal the bottom with iron sheets and tape, and transport it back to the laboratory.

[0027] (2) Indoor cultivation: place the collected algae crusts on a light culture rack for cultivation. The culture conditions are 36-54μmol·m. -2 ·S -1 , The light / dark time is 14 / 10h, spray a small amount of water every week, and each PVC tube is 50ml.

[0028] (3) Sterilization: sterilize the tweezers, dissecting needles, petri dishes, mortars, glass slides, coverslips, double-concave slides, Pasteur pipettes and sterile distilled water used for inoculation. To prepare sterile BG 11 Solid medium and pour it into 5-6 solid petri dishes.

[0029] (4) Picking of algae silk: Take the algae crust, soak it in sterile distilled...

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Abstract

The invention discloses a method for purifying and separating Microcoleus vaginatus. The method comprises the following steps of: sampling algal crusts in a desert by using a polyvinyl chloride (PVC) pipe, and carrying back to a laboratory and culturing; soaking the algal crusts in sterile distilled water in a sterilized culture dish, peeling algae of the Microcoleus vaginatus and picking out under a stereo microscope, observing and identifying the algae which are taken out under a microscope, determining that the algae are the Microcoleus vaginatus, and putting the algae into the sterile culture dish; grinding the algae of the Microcoleus vaginatus, diluting, quickly sucking out observed single algal filaments by using a Pasteur pipet under the stereo microscope, and culturing in a BG11 solid culture medium; and culturing for 2 to 3 weeks, taking out in a grown Microcoleus vaginatus colony by using a pair of sterile tweezers, performing microscopic examination under the microscope, and transferring to a sterilized BG11 liquid culture medium if the pollution is avoided. By the method, the problems of the purification and separation of the Microcoleus vaginatus are solved, and a feasible biological material is provided for crusting of desert creatures and wine protection and sand fixation; and the method has broad application fields.

Description

Invention field [0001] The present invention relates to the biological technology field of culturing and preservation of desert moss. Specifically, the present invention relates to the technical field of purification and separation of Microcodys sp. Background technique [0002] In recent years, my country’s desertification has become more and more serious, and it is one of the most severely desertified countries in the world. The total area of ​​desertified land has reached 3.73 million square kilometers with an annual expansion rate of 2,460 square kilometers. The annual direct economic loss caused by desertification is as high as 540. 100 million yuan. In view of this serious situation, it is urgent to find effective ways to prevent the desertification of land resources. [0003] Traditional wind-proof and sand-fixing measures such as afforestation, grass grid sand barriers and clay sand barriers are effective on the surface, but they cannot fundamentally solve the problems of s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCY02A40/22
Inventor 张丙昌王敬竹张元明
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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