A kind of tea tree mushroom active polysaccharide and preparation method thereof
A technology for active polysaccharide and tea tree mushroom, which is applied in the field of natural product preparation, can solve the problems of product promotion and application restriction, lack of active quality indicators, etc., and achieves the effect of promoting the phagocytic activity of mouse macrophages, processing economic benefits, and activity.
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[0022] 1) Weigh 500g of dry tea tree mushroom, after pulverization, add 1500mL of water and stir evenly. 100°C) three times (orthogonal test results see figure 1 ), centrifuge at 5000 rpm for 15 min each time for 1.5 h, discard the precipitate and keep the supernatant, concentrate the supernatant to about 1 / 4 of the original volume, add 95% ethanol 4 times the volume after concentration, and ethanol analysis for 24 h , centrifuged at 5000rpm for 15 min, discarded the supernatant, and obtained each precipitate separation sample, measured the active extract yield and anti-lipid peroxidation activity of liver tissue in each precipitate separation sample, and comprehensively evaluated to determine the optimal extraction conditions; extract and prepare the active extract, and freeze-dry to obtain the active crude polysaccharide of tea tree mushroom; the main active ingredient of the active crude polysaccharide of tea tree mushroom is 3×10 5 Acidic polysaccharide content > 40.0%, p...
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