A kind of tea tree mushroom active polysaccharide and preparation method thereof

A technology for active polysaccharide and tea tree mushroom, which is applied in the field of natural product preparation, can solve the problems of product promotion and application restriction, lack of active quality indicators, etc., and achieves the effect of promoting the phagocytic activity of mouse macrophages, processing economic benefits, and activity.

Inactive Publication Date: 2011-12-14
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Because different extraction and deproteinization processes have a great impact on the maintenance of polysaccharide biological activity, the fungal polysaccharide products currently on the market generally lack clear activity quality indicators, and the promotion and application of products are severely restricted

Method used

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  • A kind of tea tree mushroom active polysaccharide and preparation method thereof
  • A kind of tea tree mushroom active polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Weigh 500g of dry tea tree mushroom, after pulverization, add 1500mL of water and stir evenly. 100°C) three times (orthogonal test results see figure 1 ), centrifuge at 5000 rpm for 15 min each time for 1.5 h, discard the precipitate and keep the supernatant, concentrate the supernatant to about 1 / 4 of the original volume, add 95% ethanol 4 times the volume after concentration, and ethanol analysis for 24 h , centrifuged at 5000rpm for 15 min, discarded the supernatant, and obtained each precipitate separation sample, measured the active extract yield and anti-lipid peroxidation activity of liver tissue in each precipitate separation sample, and comprehensively evaluated to determine the optimal extraction conditions; extract and prepare the active extract, and freeze-dry to obtain the active crude polysaccharide of tea tree mushroom; the main active ingredient of the active crude polysaccharide of tea tree mushroom is 3×10 5 Acidic polysaccharide content > 40.0%, p...

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Abstract

The invention relates to agrocybe cylindracea active polysaccharide and a preparation method thereof. In the method, agrocybe cylindracea is used as a basic material; an activity assessment index is introduced; various indexes are comprehensively analyzed, assessed and screened through an extraction and deproteinization condition test, so that an extraction processing technology can keep the biological activity of the agrocybe cylindracea polysaccharide well and has high agrocybe cylindracea polysaccharide yield; the agrocybe cylindracea active polysaccharide and agrocybe cylindracea deproteinzed active polysaccharide are prepared under optimized technological conditions; the main active ingredient of the agrocybe cylindracea active polysaccharide for resisting peroxidization of liver tissue lipid is determined to be acidic polysaccharide with molecular weight of 300,000 through agrocybe cylindracea deproteinzed active polysaccharide sample column chromatography, active tracking and main active component molecular weight measurement; and the polysaccharide shows better biological activity for promoting mouse macrophage phagocytosis. By adopting the method, the blindness in assessment and selection of a polysaccharide extraction and separation process can be avoided; and the agrocybe cylindracea active polysaccharide prepared by taking the biological activity as the index has agood economic benefit.

Description

technical field [0001] The invention belongs to the field of natural product preparation methods, and more specifically relates to a tea tree mushroom active polysaccharide and a preparation method thereof. Background technique [0002] At present, although there are many reports on the basic research on the medicinal physiological activity of fungal polysaccharides and the discussion on the extraction process conditions of fungal polysaccharides, the research on the relevant medicinal physiological activities lacks a discussion on the relationship between the preparation conditions of the test materials and the results of the activity test; the discussion on the processing technology It is limited to optimize the extraction process with the polysaccharide yield as the evaluation index, and optimize the deproteinization process with the polysaccharide preservation rate and protein removal rate as the evaluation index. Since different extraction and deproteinization processe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P1/16
Inventor 叶舟李会霞康彦斌俞白楠吕杨兰吕千汪世华
Owner FUJIAN AGRI & FORESTRY UNIV
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