Simple industrial production method for dendrobium candidum

A technology of Dendrobium officinale and a production method, which are applied in the field of simple chemical plant production of Dendrobium officinale seedlings, can solve the problems of low yield, low survival rate of wild Dendrobium officinale seeds, slow growth and the like, and achieve carbon emission reduction, considerable economic benefits and social benefits. effect of benefit

Active Publication Date: 2011-12-21
NANKAI UNIV
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Problems solved by technology

The survival rate of wild Dendrobium officinale seeds is extremely low, and the growth is slow. It ta

Method used

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  • Simple industrial production method for dendrobium candidum
  • Simple industrial production method for dendrobium candidum
  • Simple industrial production method for dendrobium candidum

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[0023] Example

[0024] Diploid Dendrobium officinale (2n=38) was introduced from Wenshan, Yunnan Province in April 2005, such as figure 1 , The same plant cross-flower artificial pollination was carried out on May 20 of that year, and the pollinated Dendrobium officinale was at 25°C and 50% natural light until November 22, 2005. figure 2 , Cut out the capsule, sterilize it with 75% alcohol for 1 minute, then 0.1% mercury for 10 minutes, rinse with sterile water 5 times, and cut it along the longitudinal axis of the capsule with a sterile scalpel. , Inoculate the seeds of Dendrobium candidum in 1 / 2MS liquid medium and culture them with shaking at 25℃. After about 20 days, all the seeds will germinate, such as image 3 . Then transfer to 1 / 2MS solid medium for protocorm induction culture, such as Figure 4 . Figure 5 It shows that when the seed-like protocorm is differentiated, it is transferred to PP plastic bags, and each bag is inoculated with 10-15 seedlings, such as Picture...

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Abstract

The invention relates to a simple industrial production method for dendrobium candidum, which comprises the following steps: carrying out the hybrid pollination of dendrobium candidum according to the conventional method; performing the non-symbiosis germination culture of the seeds of dendrobium candidum; subjecting the protocorm-like bodies (PLBs) of the seeds to induction differentiation on a 1/2 Murashige and Skoog (MS) culture medium; inoculating the differentiated protocorms into polypropylene (PP) plastic bags and performing seedling raising differentiated culture; and transplanting seedlings 3 to 4 months later. In the invention, a new variety is provided for agriculture production in green houses in rural areas, the industrial batch production of dendrobium candidum seedlings is realized, natural heating and lighting reduce carbon emission and prevent secondary pollution, the plastic bags are suspended in the green houses for fully utilizing effective space, millions of dendrobium candidum seedlings can be grown in each mu in the green houses, and thus, the method has very considerable economic and social benefit.

Description

Technical field [0001] The invention relates to a simple chemical factory production method for Dendrobium officinale seedlings. Background technique [0002] Dendrobium candidum Wall. ex Lindl, also known as black knotweed, is an epiphytic and perennial herbaceous orchid. Mainly distributed in Yunnan, Guizhou, Guangxi, Taiwan and other places in my country. The annual average temperature is 12-18 degrees, the relative humidity is 60-75%, the forest light transmittance is about 60%, and the annual rainfall is 1100-1500mm. It grows in evergreen broad-leaved forests and is epiphytic on tree trunks or limestone. Wild Dendrobium officinale seeds have a very low survival rate and slow growth. It takes 5-7 years to harvest from seed germination to commercialization, resulting in very little yield. In recent years, the natural resources of Dendrobium officinale have been depleted due to oversupply of the market, excessive mining by people, and deterioration of the environment. There...

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 李秀兰陈力陈瑞阳
Owner NANKAI UNIV
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