Simple industrial production method for dendrobium candidum
A technology of Dendrobium officinale and a production method, which are applied in the field of simple chemical plant production of Dendrobium officinale seedlings, can solve the problems of low yield, low survival rate of wild Dendrobium officinale seeds, slow growth and the like, and achieve carbon emission reduction, considerable economic benefits and social benefits. effect of benefit
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[0024] Diploid Dendrobium officinale (2n=38) was introduced from Wenshan, Yunnan Province in April 2005, such as figure 1 , The same plant cross-flower artificial pollination was carried out on May 20 of that year, and the pollinated Dendrobium officinale was at 25°C and 50% natural light until November 22, 2005. figure 2 , Cut out the capsule, sterilize it with 75% alcohol for 1 minute, then 0.1% mercury for 10 minutes, rinse with sterile water 5 times, and cut it along the longitudinal axis of the capsule with a sterile scalpel. , Inoculate the seeds of Dendrobium candidum in 1 / 2MS liquid medium and culture them with shaking at 25℃. After about 20 days, all the seeds will germinate, such as image 3 . Then transfer to 1 / 2MS solid medium for protocorm induction culture, such as Figure 4 . Figure 5 It shows that when the seed-like protocorm is differentiated, it is transferred to PP plastic bags, and each bag is inoculated with 10-15 seedlings, such as Picture 8 , Seal the ba...
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