Protein membrane chip

A protein and membrane chip technology, applied in the field of protein chips, can solve the problems of many components, cumbersome process, unsuitable for large-scale production, etc., to avoid cross-contamination, facilitate assembly, and improve production efficiency and yield.

Inactive Publication Date: 2012-01-18
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protein chip has many components, and the membrane needs to be manually cut into

Method used

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  • Protein membrane chip
  • Protein membrane chip
  • Protein membrane chip

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Embodiment 1）

[0039] See figure 1 and figure 2 , a protein membrane chip in this embodiment includes a base 1 , a spacer 2 , a membrane 3 and an upper cover 4 .

[0040] The base 1 is an injection-molded integral part, including a base substrate 11 and a base boss 12 . The base substrate 11 is arranged horizontally, and its overall shape is a rectangle with four rounded corners. The base boss 12 is disposed at the center of the base substrate 11 , and the upper end surface of the base boss 12 is higher than the upper end surface of the base substrate 11 . Twelve fixing holes 11 - 1 are provided on the four borders of the base substrate 11 outside the base boss 12 , and each border is provided with three fixing holes 11 - 1 . The fixing holes 11-1 are all blind holes, and the three fixing holes 11-1 at each border are equally spaced.

[0041]The base boss 12 includes a base boss outer frame 12-1 and a base boss partition 12-2. The outer contour of the base boss outer frame 12-1 is a re...

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Abstract

The invention relates to a protein membrane chip which comprises a pedestal, a spacer, a diaphragm and an upper cover; a group of pedestal holes are disposed on a pedestal; a group of spacer holes are disposed on the spacer; the lower end surface of the spacer is pasted and fixed on the upper end surface of the pedestal from the upper side; the lower end surface of the diaphragm is pasted and fixed on the upper end surface of the spacer from the upper side; the upper cover comprises an upper cover substrate, an upper cover boss and a cutting edge; the upper cover boss is disposed at a center position of the upper cover substrate; the upper cover boss comprises an upper cover boss outer frame and an upper cover boss fence; the upper cover boss fence partitions a center hole of the upper cover boss outer frame into a group of upper cover holes with the same number and size as the pedestal holes; the cutting edge is disposed along the central part of the upper cover boss outer frame and the central part of the upper cover boss fence, and the upper end surface is connected to the bottom of the upper cover boss; the upper cover is detachably fixed on the pedestal; the cutting edge of the upper cover passes through the diaphragm, is inserted into the spacer, and cuts the diaphragm into protein micro-array areas. The invention is convenient for assembly, and can prevent sample cross contamination.

Description

technical field [0001] The invention relates to a biological chip, especially a protein chip. Background technique [0002] The protein membrane chip is to immobilize the protein on the membrane medium carrier in an orderly manner to make it a biochip for detection, and then perform immunodetection and analysis on it. The protein membrane chip can be detected and analyzed by chemiluminescent enzyme immunoassay. After the corresponding antigen (antibody) has an immune reaction, a solid-phase coating complex is formed. After washing, a luminescent agent is added, and the enzyme catalyzes the luminescent agent to emit light. Then, the concentration of the analyte can be calculated by collecting light signals. [0003] Chinese patent document CN2497313Y (application number 01253423.4) discloses a protein chip, which includes a box cover and a box body. It consists of a double-sided sticky gasket with holes and a plate with holes pasted under the gasket. The protein chip can b...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/76
Inventor 刘聪葛春柏海涛
Owner 上海裕隆生物科技有限公司
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